摘要
为了解广东省花生青枯病病原菌的遗传多样性,对广东省主栽花生品种近年来发生青枯病的病株进行病原菌分离,共获得64株分离菌株,其中31株为青枯菌(Ralstonia solanacearum).遗传多样性分析,发现31株青枯菌菌株均为演化型I型.随机挑取不同分离地点的花生青枯菌菌株进行致病力测定,发现来自不同地区的青枯菌致病力有所不同,其中来自湛江市的ZKRS126菌株致病力明显具有材料特异性,对花生A300致病力强而对花生A281致病力弱.此外,进化树分析的亲缘关系较近菌株致病力也有明显差异,近缘菌株ZKRS126与ZKRS206对花生A300的致病力具有显著性差异.本研究不仅有助于广东省花生青枯病的防治与研究,而且为抗青枯病花生品种的选育和研究提供基础数据和依托材料.
In order to understand the genetic diversity of the pathogen of peanut bacterial wilt in Guangdong Province, 64 strains were isolated from wilt peanut plants of main planting in Guangdong Province in recent years, among which 31 were Ralstonia solanacearum strains. Genetic diversity analysis of the 31 R. solanacearum strains demonstrated that they all belonged to phylotype I. The R. solanacearum strains from different places were randomly selected for pathogenicity analysis. The result showed that the pathogenicity of these strains was different. Among them, the pathogenicity of strain ZKRS126 from Zhanjiang was obviously variety-specific. It showed strong pathogenicity to peanut A300 but weak pathogenicity to peanut A281. In addition, the pathogenicity of strains with close genetic relationship was also significantly different. The pathogenicity of ZKRS126 and ZKRS206 to peanut A300 was significantly different. This study will contribute to the control and research of peanut bacterial wilt in Guangdong Province, and provide basic data and supporting materials for the subsequent research and breeding of resistant peanut varieties.
作者
戴小秋
陈婷
吴雨霜
杨冬
万小荣
郑奕雄
杨永
谭晓丹
DAI Xiaoqiu;CHEN Ting;WU Yushuang;YANG Dong;WAN Xiaorong;ZHENG Yixiong;YANG Yong;TAN Xiaodan(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
出处
《仲恺农业工程学院学报》
CAS
2022年第3期20-27,33,共9页
Journal of Zhongkai University of Agriculture and Engineering
基金
广东高校科研平台和项目(2021KQNCX033)
仲恺农业工程学院年薪制人员科研经费(KA210319256)
广州市特色作物种质资源研究与利用重点实验室项目(202002010010)
国家自然科学基金项目(31770652和32071737)
广东省教育厅重点领域项目资助。
关键词
花生
青枯菌
演化型
致病力
peanut
Ralstonia solanacearum
phylotype
pathogenicity
