摘要
为分离鉴定充氮包装德州扒鸡低温贮藏条件下的优势腐败菌,并选择出抑菌效果好的天然抑菌剂,将高通量测序与传统培养技术相结合,对充氮包装德州扒鸡贮藏过程中优势腐败菌进行鉴定,同时有针对性的选择四种天然抑菌剂,通过测定OD、最小抑菌浓度(MIC),研究其对德州扒鸡腐败菌的抑制效果。结果表明,德州扒鸡优势腐败菌主要为广布肉杆菌(Carnobacterium divergens)、水域微小杆菌(Exiguobacterium undae)、血嗜冷杆菌(Psychrobacter sanguinis)、变形斑沙雷氏菌(Serratia proteamaculans)、隆德假单胞菌(Pseudomonas lundensis)、溶酪大球菌(Macrococcus caseolyticus)、绿色气球菌(Aerococcus viridans)。乳酸链球菌素(Nisin)和ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)对各受试菌的抑菌能力最强,MIC约为3.91~16000μg/mL。本研究为后续Nisin和ε-PL复配在德州扒鸡防腐保鲜上的应用并延长其货架期提供了理论依据。
In order to isolate and identify the dominant spoilage bacteria of nitrogen filled packaged Dezhou-braised chicken under low temperature storage,and select the natural antibacterial agent with good antibacterial effect,taking nitrogen filled packaged Dezhou-braised chicken as the research object,the dominant spoilage bacteria in the storage process were identified by combining high-throughput sequencing with traditional culture technology.At the same time,four biological preservatives were selected,by measuring OD,and minimum inhibitory concentration(MIC) to study its inhibitory effect on spoilage bacteria of Dezhou-braised chicken.The results showed that the dominant spoilage bacteria of Dezhou-braised chicken were mainly Carnobacterium divergens、 Exiguobacterium undae、 Psychrobacter sanguinis、Serratia proteamaculans、Pseudomonas lundensis、Macrococcus caseolyticus、Aerococcus viridans.Nisin and ε-Polylysine(ε-PL) had the strongest antibacterial ability against all tested bacteria,and the MIC was about 3.91~16000 μg/mL.These results provide a theoretical basis for the subsequent application of Nisin and ε-PL combination in the preservation of Dezhou-braised chicken and the extension of its shelf life.
作者
丛筠格
梁杉
张敏
CONG Yunge;LIANG Shan;ZHANG Min(Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology&Business University,Beijing 100048,China;School of Food and Health,Beijing Technology&Business University,Beijing 100048,China)
出处
《食品工业科技》
CAS
北大核心
2022年第17期149-157,共9页
Science and Technology of Food Industry
基金
“十三五”国家重点研发计划项目(SQ2019YFC160015)。
关键词
德州扒鸡
高通量测序
菌株鉴定
优势腐败菌
乳酸链球菌素
Ε-聚赖氨酸
Dezhou-braised chicken
high-throughput sequencing
identification of strains
dominant spoilage bacteria
Nisin
ε-PL
