摘要
目的:通过16S rDNA测序研究文静汤治疗儿童抽动障碍的肠道菌群组成及变化特点,为后期相关研究奠定基础。方法:选取抽动障碍患儿共60例,随机分为两组各30例,治疗组予文静汤治疗,对照组予盐酸硫必利片治疗,评价两组临床疗效;并收集治疗组患儿粪便,采用16S rDNA扩增子测序的方法检测肠道菌群组成。结果:治疗组总有效率90.0%,高于对照组66.7%(P﹤0.05)。治疗前后患儿的肠道菌群多样性差异有统计学意义(P﹤0.05),且分布规律和数量差异均有统计学意义(P﹤0.05)。在门水平中厚壁菌门、拟杆菌门、放线菌门是患儿肠道微生物群落中的优势菌群,且治疗后放线菌门菌群丰度增加,厚壁菌门菌群丰度减少。在属水平,治疗组共有31个菌群种类的丰度在治疗后发生了明显变化,其中链球菌属及巨单胞菌属在服用文静汤治疗前后的相对变化量最大。结论:文静汤治疗儿童抽动障碍有良好的临床疗效,且服用文静汤后患儿肠道菌群的多样性、分布规律及数量等均会发生变化。
Objective:To study the composition and changing characteristics of intestinal flora of Wenjing decoction treating tic disorder of children by 16SrDNA sequencing,so as to lay a foundation for later related research.Methods:60 cases of selected children patients with tic disorder were randomly divided into two groups,30 cases in each group.The treatment group was treated with Wenjing decoction,while the control group was treated with tiapride hydrochloride tablets.The clinical efficacy was evaluated in two groups.The feces of children in the treatment group were collected,and the composition of intestinal flora was detected by 16S rDNA amplification sequencing.Results:The therapeutic efficacy of Wenjing decoction was significantly better than that of tiapride hydrochloride tables(P ﹤ 0.05).The difference of diversity of children patients’ intestinal flora before and after treatment was statistically significant(P ﹤ 0.05),and there was statistical significance in distribution law and quantity difference tablets(P ﹤ 0.05).At the phylum level,firmicutes,bacteroidetes and actinomycetes were the dominant flora in intestinal microbial community of children patients;and after treatment,the abundance of actinomycetes flora was increased to a certain extent,while the abundance of firmicutes flora was decreased to a certain extent.At the genus level,the abundance of 31 flora species in treatment group was significantly changed after treatment;among them,streptococcus and macromonas had the largest relative change before and after taking Wenjing decoction.Conclusion:Wenjing decoction has better clinical efficacy on tic disorder of children,and the diversity,distribution law and quantity etc.of intestinal flora in children patients will change after taking Wenjing decoction.
作者
项金
白晓红
XIANG Jin;BAI Xiao-hong(Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;不详)
出处
《山西中医》
2022年第9期55-58,共4页
Shanxi Journal of Traditional Chinese Medicine
关键词
儿童抽动障碍
文静汤
肠道菌群
中医药疗法
tic disorder of children
Wenjing decoction
intestinal flora
therapy of TCM
