摘要
为明晰低磷条件下内生真菌对台湾相思(Acacia confusa)叶绿素荧光特性的影响,探讨利用内生真菌提高台湾相思光合特性的可能性,从台湾相思中分离纯化4株强势内生真菌F(Filobasidium sp.)、I(Penicillium sp.)、E(Penicillium sp.)、N(Penicillium sp.)及FI混合菌(质量比1∶1),并添加于4个供磷水平(0,10,20,40 mg/kg)的土培台湾相思幼苗盆钵中.结果表明:在适量供磷条件下(40 mg/kg),对照组(未接菌种)的叶绿素含量均总体低于各菌种处理组,菌株I、FI处理组的光合系统Ⅱ(PSⅡ)活性(F_(v)/F_(o))、PSⅡ最大光能转换效率(F_(v)/F_(m))总体略高于对照组;轻度磷胁迫下(20 mg/kg),对照组的叶绿素含量始终低于菌株E、FI和F处理组,而不同处理组的F_(v)/F_(o)、F_(v)/F_(m)则总体差异不显著;重度磷胁迫下(10 mg/kg),菌株F、E、N处理组的叶绿素含量总体较高,其中菌株E处理组的F_(v)/F_(o)、F_(v)/F_(m)始终高于对照组;无磷处理下(0 mg/kg),菌株FI、I处理组在胁迫中后期的叶绿素含量较高,菌株I处理组的F_(v)/F_(o)、F_(v)/F_(m)始终略高于对照组.综上,菌株F、FI处理对台湾相思叶绿素荧光特性的促进作用最佳,明确了适合低磷环境促进台湾相思叶绿素荧光特性的菌种,可为理解内生真菌对台湾相思光合特性的促进机制提供参考,亦为沿海台湾相思海岸防护林的持续性经营提供新思路.
To clarify the effect of endophytic fungi on the chlorophyll fluorescence characteristics of Acacia confusa under low-phosphorus conditions,and to explore the possibility of using endophytic fungi to improve the photosynthetic characteristics of A.confusa,4 strains of powerful endophytic fungi F(Filobasidium sp.),I(Penicillium sp.),E(Penicillium sp.),N(Penicillium sp.)and FI mixed bacteria(1∶1)were isolated and purified from A.confusa,and added to 4 phosphorus levels(0,10,20,40 mg/kg)soil culture of A.confusa.seedlings in pots.The results showed that under an appropriate amount of phosphorus supply(40 mg/kg),the chlorophyll content of the control group(non-inoculated)was overall lower than that of each strain treatment group,and the photosynthetic systemⅡ(PSⅡ)activity(F_(v)/F_(o))and PSⅡmaximum light energy conversion efficiency(F_(v)/F_(m))of the strain I and FI treatment groups were slightly higher than those of the control group.Under mild phosphorus stress(20 mg/kg),the chlorophyll content of the control group was lower than that of the stain E,FI and F treatment groups,while the F_(v)/F_(o)and F_(v)/F_(m)were not significantly different among different treatment groups.Under severe phosphorus stress(10 mg/kg),the chlorophyll contents of the strain F,E and N treatment groups were overall higher.For the strain E treatment group,the F_(v)/F_(o)and F_(v)/F_(m)were higher than those of the control group.Under the treatment group without phosphorus(0 mg/kg),the chlorophyll contents of the strain FI and I treatment groups were higher at the middle and late stage of stress,and the F_(v)/F_(o)and F_(v)/F_(m)of the strain I treatment group were higher than those of the control group.In summary,strain F and FI show the best effect in promoting the chlorophyll fluorescence characteristics,and it is clear about the bacteria species suitable for low-phosphorus environment to promote chlorophyll fluorescence characteristics of A.confusa.These results provide a basis for understanding the promotion mechanism of endophytic fungi on photosynthetic characteristics of A.confusa,and also provide new ideas for sustainable management of A.confusa coastal protection forests.
作者
何晨阳
陈珑
王耘籽
周艳芬
李键
吴承祯
洪滔
HE Chenyang;CHEN Long;WANG Yunzi;ZHOU Yanfen;LI Jian;WU Chengzhen;HONG Tao(Key Laboratory for Forest Ecosystem Process and Management of Fujian Province,College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Ecology and Resource Engineering,Wuyi University,Nanping 354300,China)
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第5期872-879,共8页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(32071753)
国家重点研发计划(2017YFD0601304)
福建农林大学学科交叉融合推动菌草科学及产业高质量发展项目(XKJC-712021030)。
关键词
台湾相思
内生真菌
低磷
叶绿素荧光参数
Acacia confusa
endophytic fungus
low phosphorus
chlorophyll fluorescence parameter
