摘要
目的探讨miRNA-34b(miR-34b)在非小细胞肺癌(NSCLC)组织中的表达情况及其体外对人NSCLC A549细胞增殖、侵袭能力的影响。方法收集2015年6月至2017年3月山西省肿瘤医院40例NSCLC患者癌组织及癌旁正常上皮组织(距癌灶边缘5 cm以上)标本。向A549细胞分别转染miR-34b序列模拟物(实验组)和无关序列(阴性对照组)。采用反转录、实时荧光定量聚合酶链反应(qRT-PCR)法检测各组织和各组A549细胞中miR-34b表达情况;采用四甲基偶氮唑盐(MTT)法检测实验组和阴性对照组A549细胞增殖活性,Transwell法检测两组A549细胞侵袭能力。结果NSCLC组织中miR-34b相对表达量低于癌旁正常上皮组织(0.52±0.06比1.05±0.17),差异有统计学意义(P<0.001)。实验组A549细胞miR-34b相对表达量高于阴性对照组,差异有统计学意义(P<0.05)。MTT法检测显示,实验组A549细胞培养24、48h细胞增殖能力(吸光度值)均低于阴性对照组(均P<0.01)。Transwell法检测显示,实验组侵袭的A549细胞数少于阴性对照组[(49.53±5.03)个比(121.00±12.06)个,P<0.01]。结论miR-34b在NSCLC组织中低表达,miR-34b表达上调可抑制NSCLC A549细胞增殖和侵袭。
Objective To investigate the expression of miRNA-34b(miR-34b)in non-small cell lung cancer(NSCLC)tissues and its effect on proliferation and invasion of human NSCLC A549 cells in vitro.Methods The specimens of cancer tissues and paracancerous normal epithelial tissues(more than 5 cm from the edge of the tumor)were collected from 40 NSCLC patients in Shanxi Province Cancer Hospital from June 2015 to March 2017.A549 cells were transfected with miR-34b mimics(experimental group)and irrelevant sequences(negative control group),respectively.The expression of miR-34b in tissues and each group of A549 cells was detected by reverse transcription real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The proliferation activity of A549 cells in the experimental group and the negative control group was detected by methyl thiazolyl tetrazolium(MTT)assay,and the invasion ability of A549 cells in the two groups was detected by Transwell assay.Results The relative expression of miR-34b in NSCLC tissues was lower than that in paracancerous normal epithelial tissues(0.52±0.06 vs.1.05±0.17),and the difference was statistically significant(P<0.001).The relative expression of miR-34b in A549 cells of the experimental group was higher than that in the negative control group,and the difference was statistically significant(P<0.05).MTT assay showed that the cell proliferation ability(absorbance value)of A549 cells in the experimental group was lower than that in the negative control group after cultured for 24 and 48 hours(both P<0.01).Transwell assay showed that the number of invaded A549 cells in the experimental group was less than that in the negative control group[(49.53±5.03)cells vs.(121.00±12.06)cells,P<0.01].Conclusions The expression of miR-34b is low in NSCLC tissues,and the up-regulation of miR-34b expression can inhibit the proliferation and invasion of NSCLC A549 cells.
作者
苏亚峰
郭石平
廉建红
张弘广
曹国珍
杜小祥
庄晓飞
Su Yafeng;Guo Shiping;Lian Jianhong;Zhang Hongguang;Cao Guozhen;Du Xiaoxiang;Zhuang Xiaofei(The Second Clinical Medical College of Shanxi Medical University,Taiyuan 030001,China;Department of Thoracic Surgery,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China)
出处
《肿瘤研究与临床》
CAS
2022年第7期507-510,共4页
Cancer Research and Clinic
基金
山西省卫健委基金(2014049)
山西省应用基础研究计划(201801D121309)
山西省重点研发计划(201803D31171、201903D321168)。
