摘要
目的 多种分析方法联合分析枸杞多糖特性和单糖组成及木糖醇含量。方法 枸杞干果采用水提醇沉的方法提取枸杞多糖,计算提取率。建立凝胶渗透色谱-示差检测-多角度激光光散射法测定枸杞多糖分子量及分子量分布;再将提取的枸杞多糖经三氟乙酸水解为单糖,建立高效阴离子色谱-积分脉冲安培法测定水解后的阿拉伯糖、鼠李糖、半乳糖、葡萄糖、甘露糖、果糖、木糖、岩藻糖、核糖9种单糖和木糖醇。结果 采用本法检测宁夏地区的10批枸杞,枸杞多糖得率为1.86%~3.21%;10批枸杞多糖的重均相对分子量为9.12×10^(5)~3.65×10^(6)Da,离子色谱法检测10批枸杞中单糖和木糖醇含量从高到低依次为阿拉伯糖、葡萄糖、鼠李糖、半乳糖、甘露糖、果糖、木糖醇及岩藻糖。结论 本文对枸杞多糖的重均分子量及单糖组成、含量进行初步研究,与现行质量标准中检测方法相比,控制指标更加全面、客观、准确,为建立枸杞多糖的质量控制方法提供技术参考。
Objective To establish a method to jointly analyze the polysaccharides characteristics,monosaccharide composition and xylitol content of Lycium barbarum.Methods Lycium barbarumpolysaccharides were extracted from dried Lycium barbarum fruits by water extraction and alcohol precipitation,and then the extraction rate was calculated.The gel perme-ation chromatography-refractive index-multi-angle laser light scattering method was developed to determine the molecular weight and molecular weight distribution of Lycium barbarum polysaccharides. The extracted Lycium barbarum polysaccharide was hydrolyzed into monosaccharide by trifluoroacetic acid,and a high-performance anionexchange chromatography-integrating pulse amperometric detection was used to survey and evaluate nine kind of monosaccharides such as arabinose,rhamnose,galactose,glucose,mannose,fructose,xylose,fucose,ribose and one type of xylitol.Results This method was used to detect 10 batches of Lycium barbarum from Ningxia region. It was found that the yield of Lycium barbarum polysaccharide was between 1. 86% and 3. 21%. The average molecular weight of 10batches of Lycium barbarum polysaccharides was between 9. 12×105~3. 65×106Da. It was found that the order of monosaccharide and xylitol content from high to low was arabinose,glucose,rhamnose,galactose,mannose,fructose,xylitol and fucose in 10 batches of Lycium barbarum detected by ion chromatography.Conclusion This method to determine average molecular weight,monosaccharide composition and content is more comprehensive,objective and accurate than the detection method in the current quality standard. This study may provide technical reference for establishing the quality control method of Lycium barbarum polysaccharide.
作者
张玲
胡紫艳
金鑫
ZHANG Ling;HU Ziyan;JIN Xin(Nanjing Institute for Food and Drug Control,Jiangsu Nanjing 210000,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2022年第4期657-662,共6页
Chinese Journal of Food Hygiene
基金
南京市市场监督管理局科技项目(Kj2019049)。
关键词
枸杞多糖
单糖
分子量
离子色谱法
Lycium barbarumpolysaccharides
monosaccharides
molecular
ion chromatography
