摘要
目的:探讨运动对颗粒物(PM)暴露幼鼠呼吸道的p38MAPK通路的影响。方法:将幼鼠分为4组,分别为对照组、PM暴露组、PM暴露+运动干预1组和PM暴露+运动干预2组,10只/组。将3组PM暴露组的幼鼠置于加入SRM1649a(颗粒物标准品)的雾化器,雾化时间6 h/d,连续雾化12周;对照组给予磷酸缓冲盐溶液(PBS)雾化吸入。对PM暴露+运动干预的两组同期进行运动干预,运动为纵跳和游泳,其中PM暴露+运动干预2组是PM暴露+运动干预1组运动量的2倍,持续12周。雾化与运动同期开展,雾化结束,运动干预结束。对照组、PM暴露组不给予运动干预。检测运动结束后各组幼鼠的体质量、气道反应性、血清和支气管肺泡灌洗液(BALF)炎症因子[白细胞介素(IL)-1、IL-6、IL-10、肿瘤坏死因子(TNF-α)与C反应蛋白(CRP)],计算肺、脾和胸腺的脏器指数,气道行HE染色,分别采用Western Blot和免疫组化法检测气道的p38MAPK通路相关蛋白(P-p38MAPK、p38MAPK)。结果:与对照组比,PM暴露组体质量明显降低(P<0.05),肺指数、胸腺指数和脾脏指数均明显增高(P<0.05);与PM暴露组比,PM暴露+运动干预1组和PM暴露+运动干预2组体质量明显增高(P<0.05),肺指数、胸腺指数和脾脏指数均明显降低(P<0.05),且与运动干预量呈依赖性趋势。与对照组比,PM暴露组气道反应性、血清IL-1、IL-6、IL-10、TNF-α和CRP均明显增高(P<0.05);与PM暴露组比,PM暴露+运动干预1组和PM暴露+运动干预2组气道反应性、血清IL-1、IL-6、IL-10、TNF-α和CRP均明显降低(P<0.05),且与运动干预量呈依赖性降低趋势。P-p38MAPK与p38MAPK主要表达在细胞核及细胞浆。与对照组比,PM暴露组气道P-p38MAPK、p38MAPK和P-p38MAPK/p38MAPK均明显增高(P<0.05),与PM暴露组比,PM暴露+运动干预1组和PM暴露+运动干预2组P-p38MAPK、p38MAPK和P-p38MAPK/p38MAPK均明显均明显降低(P<0.05),且与运动干预量呈依赖性降低趋势。结论:颗粒物可激活幼鼠呼吸道的p38MAPK通路,给予运动干预可抑制p38MAPK通路活性,降低p38MAPK通路的P-p38MAPK、p38MAPK蛋白表达,降低炎性反应,改善肺、脾和胸腺的脏器指数。
Objective: To investigate the effect of exercise on the p38MAPK pathway in the respiratory tract of young rats exposure to particulate matter(PM). Methods: The young rats were divided into 4groups: control group, PM exposure group, PM exposure+exercise intervention group 1, and exercise intervention group 2, with 10 rats in each group. The young rats in the three PM exposure groups were placed in the nebulizer with SRM1649a(nebulized time 6 h/d) and had been nebulized continuously for 12 weeks, while the control group was given inhalation of phosphate buffer saline(PBS) solution. At the same time, exercise intervention was carried out on the two exercise intervention groups for vertical jumping and swimming. The PM exposure+exercise intervention group 2 was twice the amount of exercise in the exercise intervention group 1 and the intervention lasted for 12 weeks. Atomization and exercise intervention was carried out at the same time. The control group and the PM exposure group were not given exercise intervention. The body mass, airway responsiveness, inflammatory factors, such as serum and bronchoalveolar lavage fluid(BALF), interleukin(IL)-1, IL-6,IL-10, tumor necrosis factor alpha(TNF-α), and C reactive protein(CRP) of each group of young rats were compared. The organ coefficients of the lung, spleen, and thymus, airway pathology(assayed by HE staining), the p38MAPK pathway-related proteins levels(P-p38MAPK and p38MAPK detected by Western Blot) in the airway, and immunohistochemistry were determined in each group.Results: Compared with that respectively in control group, the body mass index of the PM exposure group had significantly reduced(P<0. 05), the lung index, thymus index,and spleen index had significantly increased(P<0. 05). Compared with that respectively in PM exposure group, the body mass index of the exercise intervention groups had significantly increased(P<0. 05), while lung index, thymus index, and spleen index had significantly decreased(P<0. 05), and in an exercise-dependent way(P<0. 05). Compared with that respectively in control group, the airway reactivity, serum and BALF IL-1, IL-6, IL-10, TNF-α and CRP in the PM exposure group had significantly increased(P<0. 05). Compared with that respectively in PM exposure group, the airway responsiveness, serum and BALF IL-1, IL-6, IL-10, TNF-α, and CRP in the two PM exposure+exercise intervention groups had significantly reduced(P<0. 05), and in an exercise-dependent way. P-p38MAPK and p38MAPK were mainly expressed in the nucleus and cytoplasm. Compared with those in control group, the airway p38MAPK and P-p38MAPK levels in the PM exposure group had significantly increased(P<0. 05), and exercise intervention could reverse the effect of PM expsoure significantly(P<0. 05), and in a way of exercise-dependence(P<0. 05). Conclusion: PM can activate the p38MAPK pathway in the respiratory tract of young rats. Exercise intervention can reduce the activity of p38MAPK, reduce expression of P-p38MAPK and p38MAPK in the p38MAPK pathway, reduce inflammatory response, and improve the organ coefficients of the lung, spleen, and thymus.
作者
吴杰妍
凌钦亮
WU Jieyan;LING Qinliang(The Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510378,Guandong,China)
出处
《武汉大学学报（医学版）》
CAS
2022年第5期709-715,共7页
Medical Journal of Wuhan University
基金
广东省中医药局科研项目(编号:20172084)。
关键词
运动
颗粒物
幼鼠
呼吸道
P38MAPK通路
影响
Exercise
Particulate Matter
Young Rats
Respiratory Tract
p38MAPK Pathway
Influence
