摘要
为探究浙江省早籼稻种质资源遗传多样性,提高早籼稻育种中亲本选择的利用效率,通过变异系数、遗传多样性指数、主成分分析、二维排序分析、逐步回归分析和聚类分析等方法对100份早籼稻种质资源的14个主要表型性状的多样性水平进行分析。结果表明:14个表型性状的变异系数在0.96%(生育期)~41.07%(每穗瘪谷数);14个表型性状的遗传多样性指数在5.50(每穗瘪谷数)~5.61(生育期);基于主成分二维排序分析筛选到单穗重大、每穗瘪谷数少、长宽比小、倒二叶长度和生育期适中、穗长长且单株有效穗多的种质资源,根据二维排序结果,早籼稻材料4128、4209、4225和4278是在二维排序重叠种质,可以作为育种亲本或者中间材料。主成分分析和综合评价表明,100份早籼稻种质资源中材料4021综合性状排名第1,筛选到每穗实粒数、每穗瘪谷数、结实率、单株有效穗、谷粒长和谷粒宽这6个表型性状可以作为早籼稻资源综合评价的关键指标;同时基于离差平方和法进行层次聚类,将100份早籼稻材料分为4类,各类表型性状差异明显。
In order to clarify the genetic diversity of early indica rice germplasm resources in Zhejiang Province and improve the utilization efficiency of parental selection in early indica rice breeding,the diversity levels of 14 main phenotypic characters of 100 early indica rice germplasm resources were analyzed by means of coefficient of variation,genetic diversity index,principal component analysis,two-dimensional ranking analysis,stepwise regression analysis and cluster analysis.The results showed that the coefficient of variation of 14 phenotypic traits ranged from 0.96%(growth period)to 41.07%(shriveld grains per panicle).The genetic diversity index of 14 phenotypic traits ranged from 5.50(shriveld grains per panicle)to 5.61(growth period).Based on the two-dimensional sorting analysis of principal components,the germplasm resources with large panicles,small number of shriveled grains per panicle,small length-width ratio,moderate length of the second leaf and moderate growth period,long panicles and more ef fective panicles per plant were screened・According to the two-dimensional sorting results,early indica rice materials 4128,4209,4225 and 4278 were overlapping germplasms in two-dimensional sorting,which could be used as breeding parents or intermediate materials.Principal component analysis and comprehensive evaluation showed that the comprehensive traits of material 4021 ranked first among 100 early indica rice germplasm resources.At the same time,six phenotypic traits,such as filled grain number per panicle,shriveled grain number per panicle,seed setting rate,effective panicle per plant,grain length and grain width,could be selected as key indicators for comprehensive evaluation of early indica rice resources.At the same time,the hierarchical clustering was conducted based on the sum of squares of deviations,and 100 early indica rice materials were divided into four categories,with significant differences in various phenotypic traits.
作者
杨海龙
王晖
雷锦超
蔡金洋
YANG Hailong;WANG Hui;LEI Jinchao;CAI Jinyang(Jiaxing Academy of Agricultural Sciences,Jiaxing 314016,Zhejiang,China;Longquan Agricultural and Rural Affairs Bureau,Longquan 323700,Zhejiang,China)
出处
《浙江农业学报》
CSCD
北大核心
2022年第8期1571-1581,共11页
Acta Agriculturae Zhejiangensis
基金
浙江省常规早籼稻新品种选育(2021C02063-4)
2016年浙江省水稻种质资源收集、繁殖(2016006)
关键词
早籼稻
种质资源
表型多样性
综合评价
early indica rice
germplasm resources
phenotypic diversity
comprehensive evaluation
