摘要
目的探讨停乳链球菌似马亚种(Streptococcus dysgalactiae subspecies equisimilis,SDSE)感染的临床分布特点及分子特征。方法收集SDSE感染患者的临床资料及相应分离菌株,分离株经全自动微生物分析系统、基质辅助激光解析电离飞行时间质谱技术(MALDI-TOF-MS)及PCR扩增16S rRNA和链激酶前体基因等3种方法进行鉴定。对SDSE菌株进行M蛋白基因(emm)分型和多位点序列分型(Multilocus sequence typing,MLST),并通过BioNumerics 6.6软件进行聚类分析。结果24株停乳链球菌似马亚种主要分离自咽拭子、皮肤、血液,分别占58.33%、20.83%、8.33%;24株SDSE被分为5种emm类型,以stCNSRT2.0(n=16,66.7%)占优势,其次是stG840.0(n=3,12.5%)。通过MLST分型,共有6种ST型别,以ST44(n=17,70.8%)为主,ST605为新定义的ST型。结论本研究中SDSE分离株具有分子多样性,ST605为首次报告的MLST型别。
To explore the clinical distribution and molecular characteristics of Streptococcus dysgalactiae subspecies equisimilis(SDSE)infection,we collected clinical data and corresponding isolates from infected patients.The isolates were identified by three methods(automatic microorganism analysis system,MALDI-TOF-MS,and PCR amplification of 16S rRNA and the streptokinase precursor gene).emm typing and multilocus sequence typing of SDSE strains were performed,and BioNumerics 6.6 software was used for cluster analysis.A total of 24 strains of SDSE were isolated primarily from throat swab(58.33%),skin(20.83%)and blood(8.33%)samples.The strains were divided into five emm types,dominated by stCNSRT2.0(n=16,66.7%),which was followed by stG840.0(n=3,12.5%).Through MLST,six sequence types were distinguished,among which ST44(n=17,70.8%)was dominant,and ST605 was a newly discovered and defined sequence type,thus indicating the molecular diversity of SDSE isolates.
作者
何放晴
李曲文
张志珊
邓艳琴
HE Fang-qing;LI Qu-wen;ZHANG Zhi-shan;DENG Yan-qin(School of Public Health,Fujian Medical University,Fuzhou 350122,China;Fujian Provincial Key Laboratory of Zoonosis Research,Fujian Center for Disease Control and Prevention,Fuzhou 350012,China;Quanzhou First Hospital,Quanzhou 362000,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2022年第7期607-613,共7页
Chinese Journal of Zoonoses
基金
国家科技重大专项(No.2018ZX10734401-007)
福建省科技创新平台建设项目(No.2019Y2001)联合资助。
关键词
停乳链球菌似马亚种
M蛋白基因分型
多位点序列分型
Streptococcus dysgalactiae subspecies equisimilis
emm typing
multilocus sequence typing
