摘要
本试验旨在探讨杂粕在生长猪结肠模拟消化与体内消化程度的差异,为完善胃-小肠-大肠三阶段仿生消化法测定猪饲料的有效能提供参考。以玉米-豆粕型饲粮为基础饲粮,菜籽粕、棉籽粕、葵花粕和花生粕分别与基础饲粮按18.75∶81.25的比例配制试验饲粮。选择5头初始体重为(39.4±2.9)kg安装有盲肠末端T型瘘管的去势公猪,采用5×5拉丁方设计,进行5期消化试验。每期均收集盲肠末端食糜和粪便,测定试验饲粮在结肠前及全消化道的干物质和能量消化率并通过套算法计算杂粕的干物质和能量消化率。生长猪结肠仿生消化采用单因素完全随机设计,基于单胃动物仿生消化系统,分别以生长猪盲肠液冻干粉剂(仿生消化法1)、模拟大肠液粉剂(仿生消化法2)制备模拟结肠液,测定试验饲粮的盲肠末端食糜在结肠的能量消化率。然后,通过胃-小肠仿生消化和胃-小肠-大(结)肠仿生消化计算仿生消化法测定杂粕在结肠的能量消化率。结果表明:1)在全消化道消化中,花生粕的能量消化率显著高于菜籽粕、棉籽粕和葵花粕(P<0.05)。4种杂粕在生长猪结肠前和结肠的能量消化率和消化能均无显著差异(P>0.05)。结肠前和结肠的能量消化率分别占全消化道能量消化率的64.05%~74.74%和25.26%~35.95%。2)仿生消化法1与体内法测定4种杂粕饲粮在结肠的能量消化率及消化能均无显著差异(P>0.05)。仿生消化法2与体内法测定的菜籽粕、棉籽粕和花生粕及相应饲粮在结肠的能量消化率和能值均无显著差异(P>0.05),但测定的葵花粕及相应饲粮在结肠的能量消化率和能值存在显著差异(P<0.05)。由此可见,仿生消化法模拟生长猪结肠对杂粕的消化程度与体内消化程度的差异受模拟消化液水解酶来源的影响。
This experiment was conducted to investigate the difference between simulated and in vivo colonic digestion of miscellaneous meal for growing pigs,providing a reference for developing a simulated digestion method to predict the available energy of feed ingredients for growing pigs.A corn-soybean meal type diet was formulated as basal diet.Eighteen-point seventy-five percent of rapeseed meal,cottonseed meal,sunflower meal or peanut meal replaced was mixed with 81.25%of basal diet,respectively,to produce 4 experimental diets.Five barrows with the initial body weight of(39.4±2.9)kg surgically fitted with T-cannulas at the terminal cecum were allotted to 5 diets for digestion experiment in a 5×5 Latin square design with 5 periods.The terminal cecal digesta and feces were collected to determine the digestibility of dry matter and energy in anterior colon and total tract of experimental diets,then the digestibility of dry matter and energy of miscellaneous meals were calculated by difference method.A single-factor completely random design was adopted to study the simulated colonic digestion of growing pigs.Based on the simulated digestion system for monogastric animal,the simulated colonic fluid was prepared with the cecal freeze-dried powder(simulated digestion method 1)or simulated intestinal fluid powder(simulated digestion method 2),respectively.The colonic energy digestibility of terminal cecal digesta of experimental diets was evaluated by simulated digestion.Then,the colonic energy digestibility of miscellaneous meals was calculated according to the gastric-small intestine and gastric-small intestine-large(colon)simulated digestion.The results showed as follows:1)total tract energy digestibility of peanut meal was significantly higher than that of rapeseed meal,cottonseed meal and sunflower meal(P<0.05).No significant differences were observed in the energy digestibility and digestible energy(DE)in anterior colon and colon of 4 miscellaneous meals(P>0.05).The energy digestibility in anterior colon and colon accounted for 64.05%to 74.74%and 25.26%to 35.95%of total tract energy digestibility,respectively.2)No significant differences were observed in the colonic energy digestibility and DE between simulated digestion method 1 and in vivo method for 4 miscellaneous meal diets(P>0.05).No significant differences were observed in the colonic energy digestibility and energetic value between simulated digestion method 2 and in vivo method for each of rapeseed meal,cottonseed meal,peanut meal corresponding diets(P>0.05),however,the significant difference was observed in sunflower meal and corresponding diet(P<0.05).It can be concluded that the difference in extent of digestion of simulated and in vivo colonic digestion of miscellaneous meal for growing pig is affected by source of simulated digestive enzymes.
作者
杜中原
王钰明
曾述礼
高理想
赵江涛
张虎
赵峰
DU Zhongyuan;WANG Yuming;ZENG Shuli;GAO Lixiang;ZHAO Jiangtao;ZHANG Hu;ZHAO Feng(State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Wen’s Food Group Co.,Ltd.,Yunfu 527400,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2022年第7期4716-4725,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
中央级公益性科研院所基本科研业务费专项资金项目(2021-YWF-ZYSQ-09)
温氏食品集团股份有限公司-中国农业科学院北京畜牧兽医研究所合作项目(2018-YF-01)。
关键词
杂粕
结肠
仿生消化
生长猪
miscellaneous meal
colon
simulated digestion
growing pigs
