摘要
本试验旨在添加棕榈油饲料中添加2种磷脂对中间球海胆生长、性腺发育及营养组成的影响。以酪蛋白、豆粕为主要蛋白质源,在鱼油饲料中添加大豆卵磷脂作为对照组饲料,在棕榈油饲料中分别添加大豆卵磷脂(PO+SL组)和磷虾油(PO+KO组)作为试验组饲料。选择初始体重的(18.86±0.55)g的中间球海胆45只,随机分为3组,分别投喂上述3种饲料,每组15只,单笼养殖。试验期42 d。结果显示:1)各组之间终末体重、增重率和性腺湿重无显著差异(P>0.05)。PO+SL组的性腺指数显著低于对照组(P<0.05)。2)PO+SL组的性腺水分含量显著高于PO+KO组和对照组(P<0.05),PO+SL组的性腺粗蛋白质含量显著低于对照组(P<0.05)。3)各组之间性腺亮度和红度值无显著差异(P>0.05)。PO+SL组的性腺黄度值显著低于PO+KO组和对照组(P<0.05)。4)性腺组织切片可见,PO+SL组的性腺发育速度最慢,雄性和雌性个体仅发育到Ⅱ期。PO+KO组和对照组的雄性个体性腺发育较快,均发育到Ⅲ期;而雌性个体性腺仅发育到Ⅱ期。5)PO+SL组的性腺总氨基酸(TAA)、必需氨基酸(EAA)、甜味氨基酸、鲜味氨基酸和苦味氨基酸含量显著高于PO+KO组和对照组(P<0.05)。6)PO+KO组和对照组的性腺中二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)含量显著高于PO+SL组(P<0.05)。综上所述,棕榈油饲料中添加大豆卵磷脂或磷虾油并未影响中间球海胆的增重率,磷虾油较大豆卵磷脂能提高中间球海胆性腺指数、发育速度及性腺必需脂肪酸含量。
This experiment was conducted to investigate the effects of two phospholipids addition in palm oilbased diet on growth,gonad development and nutritional composition of sea urchin Strongylocentrotus intermedius.The casein and soybean meal were included as the main protein sources,the soybean lecithin was added to fish oil diet as control group diet,and the soybean lecithin(PO+SL group)and krill oil(PO+KO group)were added to palm oil diets as experimental group diets,respectively.Forty-five Strongylocentrotus intermedius with initial body weight of(18.86±0.55)g were randomly allocated to 3 groups,deed the above three diets,respectively,each groups contained 15 sea urchins,and individually held in cages.The experiment lasted for 42 days.The results showed as follows:1)there was no significant difference in final body weight,weight gain rate and gonad wet weigh among all groups(P>0.05).The gonadosomatic index of PO+SL group was significantly lower than that of the control group(P<0.05).2)The gonad moisture content of PO+SL group was significantly higher than that of PO+KO group and control group(P<0.05),the gonad crude protein content of PO+SL group was significantly lower than that of the control group(P<0.05).3)There were no significant differences in gonad brightness and redness values among all groups(P>0.05).The gonad yellowness value of PO+SL group was significantly lower than that of PO+KO group and control group(P<0.05).4)The gonad tissue section showed that the gonad development rate of PO+SL group was the slowest,with both male and female individuals developed to phaseⅡ.The male individual gonad of PO+KO group and control group developed rapidly,all developed to phaseⅢ;while female individual gonad developed to phaseⅡ.5)The contents of total amino acids(TAA),essential amino acids(EAA),sweet amino acids,delicious amino acids and bitter amino acids in gonad of PO+SL group were significantly higher than those of PO+KO group and control group(P<0.05).6)The contents of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)in gonad of PO+KO group and control group were significantly higher than those of PO+SL group(P<0.05).In conclusion,the soya lecithin or krill oil addition in palm oil-based diets do not affect the weight gain rate of Strongylocentrotus intermedius.Compared with the soya lecithin,the krill oil can increase the gonadosomatic index,development rate,and essential fatty acid content of Strongylocentrotus intermedius.
作者
唐璐
贺邱雨晴
迟晓万
张峰
宁延昶
丁君
常亚青
左然涛
TANG Lu;HEQIU Yuqing;CHI Xiaowan;ZHANG Feng;NING Yanchang;DING Jun;CHANG Yaqing;ZUO Rantao(Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea,Ministry of Agriculture and Rural Affairs,College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2022年第7期4674-4688,共15页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(41606180)
中国科协青年人才托举工程(YESS20150157)。
关键词
中间球海胆
磷脂
性腺发育
生长
营养
Strongylocentrotus intermedius
phospholipid
gonad development
growth
nutrition
