摘要
针对茶叶中农药残留超标问题,应用“双链-复合物法”设计构建适配体荧光分子开关。该分子开关因啶虫脒的存在与否形成荧光信号的开与关。荧光信号强度与啶虫脒浓度对数值在2.5~500μg/mL范围内呈现良好的线性相关性(R^(2)=0.98),可实现啶虫脒的定量测定。茶叶中啶虫脒检测结果显示,测量值与理论值接近,回收率可达100.95%,进一步验证了该方法的准确性和实际应用可行性。
In the innovative experiment of “determination of acetamiprid in tea using aptamer fluorescence molecular switch”, an aptamer fluorescence molecular switch was designed and constructed by the “double strands-complex method” for the prominent problem of excess pesticide residues in tea. The fluorescence signal of this molecular switch was turned off and on depending on the presence or absence of acetamiprid. Moreover, the fluorescence intensity showed a good linear correlation(R^(2)=0.98) with the logarithm of acetamiprid concentration in the range of 2.5~500 μg/mL, which enables the quantitative determination of acetamiprid. The results of acetamiprid detection in tea show that the measured values are close to the theoretical values and the recoveries can reach 100.95%, which further verifies the accuracy of the method and the feasibility of practical applications.
作者
杨丽敏
李明明
YANG Limin;LI Mingming(College of Chemical Engineering,China University of Petroleum(East China),Qingdao 266580,Shandong,China)
出处
《实验室研究与探索》
CAS
北大核心
2022年第3期50-53,共4页
Research and Exploration In Laboratory
基金
国家自然科学基金面上项目(31871878)。
关键词
核酸适配体
荧光分子开关
啶虫脒
aptamers
fluorescence molecular switch
acetamiprid
