摘要
目的分析人脐静脉内皮细胞(HUVEC)经人羊膜间充质干细胞上清(hAMSC-CM)诱导后差异表达的circBIRC6在血管新生中的作用。方法运用实时定量PCR检测HUVEC中circBIRC6的表达水平;设计针对circBIRC6的siRNA和过表达质粒,通过转染构建敲减和过表达circBIRC6的HUVEC细胞模型;通过Transwell小室迁移实验、成管实验、创愈划痕实验检测HUVEC的血管新生能力。结果hAMSC-CM诱导HUVEC中circBIRC6的表达上升;实时定量PCR验证了HUVEC敲减和过表达circBIRC6细胞模型的可靠性;在Transwell小室迁移实验、成管实验、创愈划痕实验中,敲减circBIRC6降低了HUVEC的小室细胞迁移率、总成管长度和划痕迁移率,而过表达circBIRC6则反之。结论本研究初步阐明circBIRC6促进HUVEC血管新生的作用,并揭示hAMSC-CM可能通过上调HUVEC中circBIRC6的表达进而促进血管新生。
Objective To analyze the differentially expressed circBIRC6 in human umbilical vein endothelial cells(HUVEC)induced by conditioned medium of human amniotic mesenchymal stem cell(hAMSC-CM)and its role in angiogenesis.Methods The expression of circBIRC6 in HUVEC was tested by qRT-PCR.Si-circBIRC6 and circBIRC6-overexpressed plasmid were made to construct cell models of knockdown and overexpressed circBIRC6 by transfection.The angiogenesis ability of HUVEC was detected by Transwell migration assay,tube formation assay and wound healing assay.Results hAMSC-CM induced the expression of circBIRC6 in HUVEC.qRT-PCR verified the reliability of HUVEC knockdown and overexpression cell model.In Transwell migration assay,tube formation assay and wound healing assay,the knockdown of circBIRC6 reduced cell mobility,total tube length and scratch mobility of HUVEC,while overexpression of circBIRC6 increased them.Conclusion This study elucidates the positive role of circBIRC6 in HUVEC angiogenesis and reveals that hAMSC-CM may promote HUVEC angiogenesis by up-regulating circBIRC6.
作者
孙典
肖轶婧
沈铭
SUN Dian;XIAO Yijing;SHEN Ming(Department of General Dentistry,School of Stomatology,Nanjing Medical Universily,Nanjing 210029 China)
出处
《口腔医学》
CAS
2022年第6期508-514,共7页
Stomatology
基金
国家自然科学基金(81400535)。
