摘要
主要讨论及建立SMART柱在线自动净化-高效液相串联荧光检测法同时检测黄曲霉毒素(AF)M_(1)、M_(2)的方法。以50℃热水溶解样品,以75%甲醇水溶液震荡提取样品,免疫亲和柱自动净化,用Waters Bridge C_(18)柱(4.6 mm×150 mm,2.5μm)分离测定,流动相为V_(水)︰V_(甲醇)︰V_(乙腈溶液)=70︰15︰15,流速0.6 mL/min,激发波长360 nm,发射波长430 nm,色谱柱温度30℃。结果表明,AFM_(1)、AFM_(2)在0.05~5 ng/mL范围内线性良好,相关系数均大于等于0.999。该方法简单快捷、重复性好、灵敏度高,且降低试验中人员所承担的检测风险。
Mainly discuss and establish a SMART column online automatic purification-high performance liquid phase tandem fluorescence detection method to simultaneously detect aflatoxin(AF)M_(1),M_(2).In this method,the sample was dissolved in hot water at 50℃,and then shaken with 75% methanol aqueous solution to extract the sample.The immunoaffinity column was automatically purified,and the waters bridge C_(18) column(4.6 mm×150 mm,2.5μm)was used for separation and determination.The mobile phase was V_(water)︰V_(methanol)︰V_(acetonitrile)=70︰15︰15,flow rate 0.6 mL/min,excitation wavelength 360 nm,emission wavelength 430 nm,column temperature 30℃.It was concluded that AFM_(1) and AFM_(2) had good linearity in the range of 0.05-5 ng/mL,and the correlation coefficients were all greater than or equal to 0.999.The method was simple,fast,reproducible,and highly sensitive,and reduces the detection risk borne by the experimenters.
作者
倪鲁波
唐雷鸣
陆羚子
戴意飞
刘婷
蒋玲波
NI Lubo;TANG Leiming;LU Lingzi;DAI Yifei;LIU Ting;JIANG Lingbo(Zhoushan Institute for Food and Drug Control,Zhoushan 316000)
出处
《食品工业》
CAS
2022年第4期314-316,共3页
The Food Industry
基金
浙江省市场监督管局科研计划项目(20190351)。
关键词
液相荧光检测
黄曲霉毒素
乳粉
全自动净化处理
liquid phase fluorescence detection
aflatoxin
milk powder
automatic purification treatment
