摘要
目的建立小鼠星状神经节阻滞(SGB)模型,并探讨SGB对脑皮质血流量的影响。方法清洁级健康雄性C57BL/6小鼠30只,8~9周龄,体重23~27 g,采用随机数字表法分为5组(n=6):对照组(C组)、左侧SGB组(L组)、左侧生理盐水组(SL组)、右侧SGB组(R组)及右侧生理盐水组(SR组)。C组不予任何处理;L组和R组采用0.25%罗哌卡因0.08 ml行经皮后入路SGB;SL组和SR组注射等量生理盐水。于SGB前(T_(0))及SGB后10、30、60、90、120 min(T_(1~5))时,采用激光散斑血流成像系统检测脑皮质血流量。结果L组和R组小鼠均出现阻滞侧上睑下垂,提示小鼠SGB模型制备成功。C组、SL组和SR组各时点脑皮质血流量差异无统计学意义(P>0.05),L组和R组阻滞侧脑皮质血流量T_(1)时降低,T_(2)时开始升高,T_(3)时达峰值,T_(5)时降低,但仍高于T_(0)时(P<0.01);与C组和SL组比较,L组左侧脑皮质血流量T_(1)时降低,T_(2~5)时升高(P<0.01);与C组和SR组比较,R组右侧脑皮质血流量T_(1)时降低,T_(2~5)时升高(P<0.01);C组与SL组间、C组与RL组间各时点脑皮质血流量差异无统计学意义(P>0.05)。结论本研究成功建立了小鼠经皮后入路SGB模型。单侧SGB可对阻滞侧脑皮质血流量产生影响,表现为短暂降低之后持续性升高。
Objective To develop a model of stellate ganglion block(SGB)in mice and investigate the effect of SGB on cerebral cortical blood flow.Methods Thirty clean-grade healthy male C57BL/6 mice,aged 8-9 weeks,weighing 23-27 g,were divided into 5 groups(n=6 each)using a random number table method:control group(group C),left SGB group(group L),left normal saline group(group SL),right SGB group(group R)and right normal saline group(group SR).Group C received no intervention.SGB was performed with 0.25%ropivacaine 0.08 ml via percutaneous posterior approach in L and R groups,while the equal volume of normal saline 0.08 ml was given instead at the location of left and right stellate ganglion in SL and SR groups,respectively.The cerebral cortical blood flow was determined using laser speckle contrast imaging system before SGB(T_(0))and at 10,30,60,90 and 120 min after SGB(T_(1-5)).Results Mice developed ptosis on the block side,indicating that the model of SGB was successfully developed in L and R groups.There was no significant difference in cerebral cortical blood flow at each time point among C,SL and SR groups(P>0.05),and cerebral cortical blood flow on the block side decreased at T_(1),began to increase at T_(2),peaked at T_(3),and decreased at T_(5) which was still higher than that at T_(0) in group L and group R(P<0.01).Compared with C and SL groups,the left cerebral cortical blood flow was significantly decreased at T_(1,5) and increased at T_(2-4) in group L(P<0.01).Compared with C and SR groups,the right cerebral cortical blood flow was significantly decreased at T_(1,5) and increased at T_(2-4) in group R(P<0.01).There were no significant differences in cerebral cortical blood flow at each time point between group C and group SL and between group C and group RL(P>0.05).Conclusions The mouse model of SGB via percutaneous posterior approach is successfully developed.Unilateral SGB can affect cerebral cortical blood flow on the block side,which shows a transitory decrease followed by a sustained significant increase.
作者
王嘉华
周炜
王小洪
严诗婷
田顺平
王莹
于乐洋
李虎
张冬生
张转
刘微丽
Wang Jiahua;Zhou Wei;Wang Xiaohong;Yan Shiting;Tian Shunping;Wang Ying;Yu Le-yang;Li Hu;Zhang Dongsheng;Zhang Zhuan;Liu Weili(Department of Anesthesiology,the Affiliated Hospital of Yangzhou University,Yangzhou 225012,China;School of Medicine,Yangzhou University,Yangzhou 225009,China;Graduate School,Dalian Medical University,Dalian 116000,China;Department of Intensive Care,the Affiliated Hospital of Yangzhou University,Yangzhou 225012,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2022年第4期430-434,共5页
Chinese Journal of Anesthesiology
基金
扬州市卫生和计划生育委员会基金医学重点人才培养项目(ZDRC201815)
扬州大学附属医院院级课题重点项目(YZYY2017-07)。
关键词
星状神经节
神经传导阻滞
模型
动物
脑血管循环
大脑皮质
Stellate ganglion
Nerve block
Models,animal
Cerebrovascular circulation
Cerebral cortex
