摘要
为深入了解饵料微藻中类胡萝卜素在双壳贝类生长发育阶段中的吸收和代谢过程,本试验选择我国重要的滩涂养殖贝类——厚壳贻贝(mytilus coruscus)作为研究对象,采用液相色谱-质谱联用技术(HPLC-MS)分析饵料微藻喂养后的幼虫阶段——担轮幼虫、D形幼虫、壳顶期、眼点期和稚贝期中类胡萝卜素分布和代谢变化。结果表明,在9种饵料微藻中,叉鞭金藻和牟氏角毛藻的总类胡萝卜素含量最高,分别达到1 290.7和1 326.4 mg·kg^(-1)。外源摄入这两种饵料微藻后,幼虫阶段中共鉴定出5种类胡萝卜素,包括1种新合成类胡萝卜素(贻贝黄素)和4种微藻来源类胡萝卜素(α-胡萝卜素、β-胡萝卜素、岩藻黄酮醇、岩藻黄素),其中岩藻黄素的含量最高,与微藻中岩藻黄素含量高的现象一致。担轮幼虫中未检测到类胡萝卜素,而贻贝黄素仅从壳顶期开始检出。类胡萝卜素的变化与发育阶段呈正相关,从D形幼虫到壳顶期,岩藻黄酮醇、岩藻黄素、α-胡萝卜素和β-胡萝卜素的累积最为迅速,分别增加了20.3、20.3、8.0和1.5倍,并在稚贝期达到最高值。此外,与类胡萝卜素吸收代谢相关的基因ABCA1、SRB1和βCMOOX的表达量均显著上调。其中,参与合成代谢的βCMOOX表达最显著,从壳顶期到稚贝期,其表达量增加了38.2~43.3倍,表明该基因可能参与了壳顶期贻贝黄素的合成。本研究结果为了解贝类生物中类胡萝卜素的生物合成过程及代谢途径提供了基础数据。
To understand the metabolic transformation of carotenoids between the dietary microalgae and the growth and development of shellfish, different larval stages of Mytilus coruscus were investigated by feeding with microalgae. High performance liquid chromatography coupled with mass spectrometry(HPLC-MS) was utilized for carotenoids profiling among trochophore, D-shaped larvae, shell stage, eye point phase and juvenile of M. coruscus. The contents of total carotenoids were 1 290.7±111.3 mg·kg^(-1) and 1 326.4±117.1 mg·kg^(-1) in Chaetoceros mulleri and Dicrateria inornate, respectively, which were higher than other microalgae. After feeding by the mixture of C. mulleri and D. inornate, five kinds of carotenoids were detected, including α-carotene, β-carotene, fucoxanthinol, fucoxanthin, and mytiloxanthin, a new synthetic carotenoid in larval stage. The high content of fucoxanthin in M. coruscus was consistent with that in the microalgae. There was no carotenoid found in trochophore, while mytiloxanthin was initial observed since the shell stage of juvenile. A positive correlation was found between the contents of carotenoids and growth stage. The increments of fucoxanthinol, fucoxanthin, α-carotene and β-carotene were fast in D-shaped larvae, increased by about 20.3, 20.3, 8.0 and 1.5 folds when compared to those of shell stage and the contents of carotenoids reached the maximum in juvenile. Besides, the expressions of related genes(ABCA1, βCMOOX, and SR-BI) were upregulated at different larval stages, especially βCMOOX gene, which participated in the biosynthesis of carotenoids. The levels of βCMOOX increased rapidly from D-shaped larva to shell stage about 38.2~43.3 folds, which suggested that βCMOOX might be involved in the synthesis of mytiloxanthin during the shell stage. The results provided data support for elucidation of the metabolism pathway of carotenoids in shellfish.
作者
翁梓雨
李双
陈娟娟
WENG Ziyu;LI Shuang;CHEN Juanjuan(Ningbo University,State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo,Zhejiang 315211;Ningbo Customs Technology Center,Ningbo,Zhejiang 315040)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第7期1402-1412,共11页
Journal of Nuclear Agricultural Sciences
基金
国家重大专项(2018YFD0901103)
宁波市公益科技项目(2021S063)
国家现代农业产业技术体系(CARS-50)
宁波大学王宽诚幸福基金。
关键词
厚壳贻贝
幼虫
类胡萝卜素
饵料微藻
高效液相色谱-质谱法(HPLC-MS)
Mytilus coruscus
larval stages
carotenoids
dietary microalgae
high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)
