摘要
绿色超级稻的育种目标是少打农药、少施化肥、节水抗旱和优质高产。为培育“少打农药,节水抗旱”的资源节约型、环境友好型水稻品种,本研究以T1C-19、T2A-1作为cry1C^(*)和cry2A的基因供体,与节水抗旱稻恢复系旱恢3号品种杂交,利用分子标记辅助选择技术对目标基因进行筛选,最终获得同时含有cry1C^(*)和cry2A基因的稳定株系。通过PCR以及对Cry1C^(*)和Cry2A蛋白的定性和定量检测,结合正常管理田间农艺性状考察及不防治螟虫田间管理的抗虫性鉴定,筛选出双价抗虫节水抗旱稻新恢复系旱恢3CA。结果显示,对照旱恢3号与旱恢3CA在农艺性状上无显著差异,但早恢3CA高抗稻纵卷叶螟。本研究结果为培育既节水抗旱又抗虫的水稻新品种奠定了材料基础。
Great reduction the consumption of pesticides,chemical fertilizers,water,and high grain yield,superior quality,are the breeding objects of the Green Super Rice.In order to breed resource-conserving and environment-friendly rice varieties with“less pesticide,and water saving and drought resistance”,T1 C-19 and T2 A-1 rice lines were used as cry1C^(*)and cry2A gene donors to cross with Hanhui No.3,a water-saving and drought resistant rice restorer line.The target genes were screened by molecular marker assisted selection(MAS)technology,and finally stable lines containing both cry1C^(*)and cry2A gene were obtained.Through PCR detection of cry1C^(*)and cry2A genes,qualitative and quantitative detection of Cry1 C^(*)and Cry2 A proteins,combined with the investigation of agronomic traits in the field under normal management and the identification of insect resistance in field management without control of stem borers,a new bivalent insect-resistant,water-saving and drought-resistant rice restorer line named Hanhui 3 CA was screened out.The results showed that compared with the control plant of Hanhui No3,Hanhui 3 CA had no significant difference in agronomic traits,but with high resistance to rice leaf roller.The results of this study laid a material foundation for the cultivation of new insect resistance rice varieties with water saving and drought resistance.
作者
叶水烽
高宁宁
陈魅瑶
曾海云
马国华
YE Shuifeng;GAO Ningning;CHEN Meiyao;ZENG Haiyun;MA Guohua(College of Life Sciences,Shangrao Normal University,Shangrao,Jiangxi 334001;Southern Zhejiang Key Laboratory of Crop Breeding,Wenzhou Vocational College of Science and Technology(Wenzhou Academy of Agricultural Sciences),Wenzhou,Zhejiang 325006;Shanghai Agrobiological Gene Center,Shanghai201106)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第7期1300-1307,共8页
Journal of Nuclear Agricultural Sciences
基金
转基因生物新品种培育重大专项(2016ZX08001001-001)
温州市基础性科研项目(N2020002)
浙南作物育种重点实验室开放课题研究基金(2020SZCB02)。
关键词
双价
抗虫
节水抗旱稻
杂交
bivalent
insect-resistance
water-saving&drought-resistance rice
hybrids
