摘要
【背景】投加微生物菌剂是强化生物处理效能的重要手段,反硝化是污水脱氮除磷的关键步骤,但目前对于反硝化微生物菌剂相关的研究报道较少。【目的】驯化高效反硝化聚磷菌菌剂,并对系统进行生物强化。【方法】采用两阶段法快速富集反硝化聚磷菌,筛选高效脱氮除磷功能菌株NC1-1并进行鉴定,以NC1-1为菌种来源制备干粉菌剂,研究菌剂强化A^(2)SBR系统污水处理效果。【结果】历经36 d后反硝化聚磷菌富集成功,菌株NC1-1经鉴定属于戈登氏菌属,其脱氮除磷率分别为89.46%和91.68%。麦麸、玉米粉配比为85%:15%、NC1-1投菌量为20 mL、发酵液用量20 mL的条件下成功制得干粉菌剂,干粉菌剂最佳投加量为10%的A^(2)SBR系统总磷(total phosphorus,TP)和NO_(3)^(-)-N去除率比未投加菌剂的A^(2)SBR系统提高12.06%和11.52%。【结论】菌剂NC1-1的投加使A^(2)SBR系统的污染物去除效能进一步提高,研究结果为进一步研究反硝化聚磷菌菌剂提供了理论依据。
[Background]Adding microbial inoculum is an important means to enhance the efficiency of biological treatment,and denitrification is a key step in nitrogen and phosphorus removal from wastewater.However,there are few reports on denitrifying microbial agents.[Objective]To acclimate efficient denitrifying phosphorus accumulating organism(DPAO),make the denitrifying agent,and enhance the agent performance.[Methods]The DPAO was rapidly enriched by two-phase method,and strain NC1-1 with high-efficiency nitrogen and phosphorus removal was screened out and identified.The bacterial powder was prepared with NC1-1 strain,and the effect of the powder on wastewater treatment in A^(2)SBR system was studied.[Results]DPAO was enriched successfully after 36 days.Strain NC1-1 was identified as Gordonia,with the nitrogen and phosphorus removal rates of 89.46%and 91.68%,respectively.The bacterial powder was successfully prepared with the wheat bran-to-corn flour ratio of 85%:15%,NC1-1 inoculum volume of 20 mL,and fermentation broth volume of 20 mL.The removal rates of total phosphorus(TP)and NO_(3)^(-)-N in the A^(2)SBR system supplemented with the bacterial powder at a dosage of 10%were 12.06%and 11.52%,respectively,higher than those in the A^(2)SBR system without the bacterial powder.[Conclusion]The adding of NC1-1 enhanced the pollutant removal efficiency of A^(2)SBR system.The research outcome provides a theoretical basis for the further study of DPAO agent.
作者
李微
王宇琦
侯云鹤
王贺
陈一鸣
高明杰
祝雷
LI Wei;WANG Yuqi;HOU Yunhe;WANG He;CHEN Yiming;GAO Mingjie;ZHU Lei(Shenyang Jianzhu University,Shenyang 110015,Liaoning,China;Liaoning Urban and Rural Construction Planning and Design Institute Company Limited,Shenyang 110015,Liaoning,China;Shenyang Academy of Environmental Sciences,Shenyang 110015,Liaoning,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2022年第6期2022-2036,共15页
Microbiology China
基金
辽宁省教育厅自然科学基金(lnqn202010)
辽宁省科技厅自然科学基金(2019ZD0671)。
