摘要
大肠杆菌对喹诺酮类耐药性逐年上升给人畜健康带来威胁.利用低能N^(+)注入诱变大肠杆菌,通过测定诱变菌株耐药谱和最小抑菌浓度(MIC),结合16S rRNA序列差异分析,研究低能N^(+)注入对大肠杆菌耐药性的影响.结果筛选出5株对喹诺酮类耐药的菌株,其对诺氟沙星的MIC增加了16~32倍,16S rRNA的突变率达到0.8%~3.6%.研究表明低能N^(+)注入可能通过引发16S rRNA基因突变,从而改变大肠杆菌的耐药性能,这为探索大肠杆菌的耐药发生机制提供了参考.
Quinolones resistance of Escherichia coli is increasing year by year,which poses a threat to human and animal health.In this study,the drug resistance spectrum,MIC and the 16S rRNA sequence analysis was used to study the effect of low energy N^(+)implantation on the drug resistance of E.coli.The results showed that five quinolones resistant strains were screened,of which the MIC of norfloxacin increased 16~32 times and the mutation rate of 16S rRNA were 0.8%~3.6%.Low energy N^(+)implantation might change the drug resistance of E.coli by inducing 16S rRNA gene mutation,which provides a reference for exploring the mechanism of drug resistance of E.coli.
作者
王婷
张敏
李天宇
钱卫东
唐朝
蔡长龙
WANG Ting;ZHANG Min;LI Tian-yu;QIAN Wei-dong;TANG Chao;CAI Chang-long(College of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi′an 710021, China;School of Optoelectronic Engineering, Xi′an University of Technology, Xi′an 710021, China)
出处
《陕西科技大学学报》
北大核心
2022年第4期61-66,共6页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金项目(11975177)
陕西省西安市科技计划项目(201921654GXRC001CG002-GXYD1.6)。
