摘要
目的:建立高压液相色谱法(HPLC)同时分析并测定泌感合剂中落新妇苷、野黄芩苷、野黄芩素、木犀草素、芹菜素、异鼠李素的含量。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6×250 mm,5μm);流动相:甲醇(A)-0.4%磷酸水(B),梯度洗脱;检测波长320 nm;流速1.0 mL/min;柱温23℃。结果:落新妇苷、野黄芩苷、野黄芩素、木犀草素、芹菜素、异鼠李素分别在0.144~1.44μg(r=0.9999)、0.196~1.96μg(r=0.9997)、0.076~0.76μg(r=0.9993)、0.034~0.34μg(r=0.9993)、0.032~0.32μg(r=0.9998)、0.026~0.26μg(r=0.9995)范围内进样量与峰面积线性关系良好;平均回收率在分别为95.6%、96.3%、94.5%、92.3%、97.8%、95.2%;相对标准偏差(RSD)分别为1.7%、1.5%、2.2%、1.2%、2.0%、1.6%。结论:该方法操作简单、稳定、可重复,可用于同时分析测定泌感合剂中6种黄酮类成分的含量。
Objective:To develop a method for simultaneous determination of astilbin,scutellarin,scutellarein,luteolin,apigenin,and isorhamnetin in Migan Mixture by high-performance liquid chromatography(HPLC).Methods:Separation was performed on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5μm)at 23℃.The mobile phases were methanol(A)-0.4%phosphoric acid(B)with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 320 nm.Results:Astilbin,scutellarin,scutellarein,luteolin,apigenin,and isorhamnetin showed good linearity between the injection volume and the peak area at 0.144~1.44μg(r=0.9999),0.196~1.96μg(r=0.9997),0.076~0.76μg(r=0.9993),0.034~0.34μg(r=0.9993),0.032~0.32μg(r=0.9998),and 0.026~0.26μg(r=0.9995),respectively.The average recoveries were 95.6%,96.3%,94.5%,92.3%,97.8%,and 95.2%,with RSDs of 1.7%,1.5%,2.2%,1.2%,2.0%,and 1.6%,respectively.Conclusion:This method is simple,stable,and repeatable,which can be used to determine the content of six flavonoids in Migan Mixture by HPLC.
作者
彭敏
符映均
童晓东
姜晓燕
杨水英
刘忠美
倪红辉
张硕
PENG Min;FU Yingjun;TONG Xiaodong;JIANG Xiaoyan;YANG Shuiying;LIU Zhongmei;NI Honghui;ZHANG Shuo(Nantong Hospital of Traditional Chinese Medicine,Jiangsu Province,Nantong 226000,China)
出处
《世界中医药》
CAS
2022年第10期1368-1370,1376,共4页
World Chinese Medicine
基金
江苏省卫生健康委项目2021年度医学科研项目(Z2021081)
南通市中医医疗联盟课题(TZYK201811,TZYK201803)。
关键词
泌感合剂
落新妇苷
野黄芩苷
野黄芩素
木犀草素
芹菜素
异鼠李素
高压液相色谱法
含量测定
Migan Mixture
Astilbin
Scutellarin
Scutellarein
Luteolin
Apigenin
Isorhamnetin
High-performance liquid chromatography
Simultaneous determination
