摘要
该实验采用超高效液相色谱串联四极杆飞行时间质谱(UPLC-Q-TOF-MS^(E))技术,对不同批次黄连的地上部分和地下部分进行化学成分比较研究。通过与对照品的保留时间、分子离子峰和LC-MS^(E)碎片信息进行比对或参考现有文献资料,共指认了40个成分,从黄连主根中指认了33个化合物,黄连茎叶中指认了31个化合物,二者共有成分24个,主要为小檗碱、药根碱等生物碱类及绿原酸、奎宁酸、丹参素等酚酸类化合物,这表明黄连茎叶中有较多化学成分与主根相同。同时两者也存在差异成分,黄连主根中含有木兰花碱葡萄糖苷等生物碱糖苷,黄连茎叶含有(±)lariciresionol-4-β-D-glucopyranoside等木脂素类化合物,通过与对照品二级谱图比对进行裂解规律分析。不同批次间黄连化合物含量存在差异,进一步进行主成分分析(PCA)结果表明,黄连的主根和茎叶清晰地聚成2类,说明各部位所含化学成分存在差异。进一步开展偏最小二乘法判别分析(PLS-DA),结果表明黄连的主根和茎叶中VIP>1的化合物有31个,差异成分主要为生物碱类、酚酸类、木脂素类以及黄酮类化合物等。研究结果表明,黄连茎叶中可利用成分种类较多,具有良好的开发潜力。该研究为黄连非药用部位的开发利用提供数据支撑,也为其他中药非药用部位的综合开发利用提供思路。
The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS^(E))technology was employed to compare the chemical components between the aerial and underground parts of Coptis chinensis samples from different batches.According to the retention time,molecular ion peak,and LC-MS^(E)fragment information of the reference substances and available literature,we identified a total of 40 components.Thirty-three and 31 compounds were respectively identified in the underground part(taproots)and the aerial part(stems and leaves)of C.chinensis.Among them,24 compounds,including alkaloids(e.g.,berberine and jatrorrhizine)and phenolic acids(e.g.,chlorogenic acid,quinic acid,and tanshinol),were common in the two parts.In addition,differential components were also identified,such as magnoline glucoside in the underground part and(±)lariciresionol-4-β-D-glucopyranoside in the aerial part.The analysis of fragmentation pathways based on spectra of reference substances indicated the differences among samples of different batches.Furthermore,we performed the principal component analysis(PCA)for the peak areas of C.chinensis in different batches.The results showed that the underground part and the aerial part were clearly clustered into two groups,indicating that the chemical components contained in the two parts were different.Furthermore,the results of partial least squares discriminant analysis(PLS-DA)identified 31 differential compounds(VIP value>1)between the underground part and the aerial part,mainly including alkaloids,phenolic acids,lignans,and flavonoids.This study proves that C.chinensis possesses great development potential with multiple available compounds in stems and leaves.Moreover,it sheds light on for the development and utilization of non-medicinal organs of C.chinensis and other Chinese medicinal herbs.
作者
朱童
杨丹
刘珊珊
滕菲
朱晶晶
张永欣
徐国兵
刘守金
王智民
陈两绵
高慧敏
ZHU Tong;YANG Dan;LIU Shan-shan;TENG Fei;ZHU Jing-jing;ZHANG Yong-xin;XU Guo-bing;LIU Shou-jin;WANG Zhi-min;CHEN Liang-mian;GAO Hui-min(School of Pharmary,Anhui University of Chinese Medicine,Hefei 230012,China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Analysis and Testing,Beijing Academy of Science and Technology(Beijing Center for Physical and Chemical Analysis),Beijing 100089,China;Anhui Institute for Food and Drug Control,Hefei 230051,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第4期980-987,共8页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2017YFC1701900)
北京市改革与发展专项(2021ZL0119)。
关键词
黄连
中药非药用部位
质谱指认
Coptis chinensis
non-medicinal organs of Chinese medicinal herbs
mass spectrometry-based identification
