摘要
丛枝菌根(arbuscular mycorrhizae,AM)真菌作为专性寄生菌,可与植物共生并发挥生态功能,为探究植物根系结构特征对AM真菌多样性的影响,以东北林业大学校园内的二月兰(Orychophragmus violaceus)、八宝景天(Sedum spectabile)、狼尾草(Pennisetum alopecuroides)和肥皂草(Saponaria officinals)为材料,分析4种植物的根长、表面积、投影面积、平均直径、总体积、根尖数、分枝数和交叉数,测定根围土的理化性质,分离根围土中的孢子并进行形态鉴定。结果表明:4种植物根围共鉴定到AM真菌6属27种,其中狼尾草根围鉴定出5属15种,为共生AM真菌种最丰富的植物,波罗的海内养囊霉(Entrophospora baltica)分布于每种植物的根围土中,对不同植物根围AM真菌多样性的影响较小。根系特征影响了植物根围AM真菌的分布,且根表面积和投影面积所发挥的影响力最大。研究结果可为了解AM真菌多样性与植物根系特征的关系提供理论依据。
Arbuscular mycorrhizal(AM)fungi,as an obligate parasitic fungi,can form symbiosis with plants and play ecological functions.To explore the effects of root characteristics on AM fungal diversity,the rhizosphere soils of Orychophragmus violaceus,Sedum spectabile,Pennisetum alopecuroides,and Saponaria officinals growing in Northeast Forestry University were used as materials.The spores in the soil were separated and then identified.The roots of the four plants were scanned to determine their length,superficial area(SA),projected area(PA),average diameter(AvgD),volume(Vol),tip number(NTips),forks number(NForks),and number of cross(NCross).In addition,the physicochemical properties of the rhizosphere soil were measured to determine the conditions of the sampling sites.The results showed that 27 species belonged to 6 genera of AM fungi.Among them,15 species belonged to 5 genera that were detected in the rhizosphere of P.alopecuroides,indicating that P.alopecuroides was the species with the most abundant AM fungal resources.Entrophospor baltica was distributed in the rhizosphere of each plant and it exerted little effect on AM fungal composition among the different plants.Root characteristics affected the distribution of AM fungi,and SA and PA played the greatest impact.These results will provide a theoretical basis for understanding the relationship between AM fungal diversity and root characteristics.
作者
刘雅洁
王玉丹
曹红月
王琦玲
李海薇
阚佳莹
杨春雪
LIU Yajie;WANG Yudan;CAO Hongyue;WANG Qiling;LI Haiwei;KAN Jiaying;YANG Chunxue(College of landscape architecture,Northeast Forestry University,Harbin 150040,Heilongjiang,China)
出处
《草业科学》
CAS
CSCD
北大核心
2022年第6期1185-1196,共12页
Pratacultural Science
基金
国家自然科学基金青年科学基金项目(31601986)
黑龙江省博士后科研启动金(LBH-Q16005)。
关键词
AM真菌多样性
根系扫描
校园绿地
二月兰
肥皂草
狼尾草
八宝景天
AM fungal diversity
roots scanning
campus landscape
Orychophragmus violaceus
Saponaria officinals
Pennisetum alopecuroides
Sedum spectabile
