摘要
核氧还蛋白(nucleoredoxin,NRX)可通过还原目标蛋白的二硫键来调控其生物活性,在植物的生长发育和抗逆境胁迫中发挥着重要作用。蛋白质二硫键异构酶(protein disulfide isomerase,PDI)、h型硫氧还蛋白(h-type thioredoxin,TRXh)和蛋白磷酸酶2A催化亚基(protein phosphatase 2A catalytic subunit,PP2Ac)是小麦核氧还蛋白TaNRX1的互作蛋白。为了明确TaNRX1互作蛋白的抗旱性功能,本研究在拟南芥中过表达了小麦TaPDI-A、TaTRXh-A和TaPP2Ac-D基因,对野生型和转基因拟南芥的表型和抗旱相关生理指标进行了鉴定。结果表明,干旱胁迫处理后,转TaPDI-A、TaTRXh-A和TaPP2Ac-D基因拟南芥的根长、存活率、脯氨酸含量均大于野生型,离体叶片失水率、丙二醛(MAD)含量均小于野生型。二氨基联苯胺(diaminobenzidine,DAB)对H_(2)O_(2)组织定位染色结果表明,干旱胁迫处理后,转TaPDI-A、TaTRXh-A和TaPP2Ac-D基因拟南芥的H_(2)O_(2)含量均低于野生型。上述结果说明,TaNRX1的互作蛋白基因TaPDI-A、TaTRXh-A和TaPP2Ac-D增强了拟南芥对于干旱胁迫的抵抗能力。本研究可为小麦抗旱育种提供候选基因和理论基础。
Nucleoredoxin(NRX)may regulate its biological activity by reducing the disulfide bond of the target proteins,which plays an important role in plant growth,development and stress resistance.Protein disulfide isomerase(PDI),h-type thioredoxin(TRXh)and protein phosphatase 2A catalytic subunit(PP2Ac)are interaction proteins of wheat TaNRX1 protein.In order to clarify the drought resistance function of TaNRX1 interaction proteins,in this study,we overexpressed the wheat TaPDI-A,TaTRXh-A and TaPP2Ac-D genes in Arabidopsis thaliana,and characterized phenotypes and drought resistance related physiological indices of wild-type and transgenic Arabidopsis thaliana.The results showed that under drought stress,the root length,survival rate and proline content of transgenic Arabidopsis with TaPDI-A,TaTRXh-A and TaPP2Ac-D genes were all higher than those of wild-type;The water loss rate of detached leaves and malondialdehyde(MAD)content were lower than those of wild-type.And the results of hydrogen peroxide(H_(2)O_(2))localization by diaminobenzidine(DAB)staining showed that under drought stress,the H_(2)O_(2) content of Arabidopsis thaliana with overexpression of TaPDI-A,TaTRXh-A and TaPP2Ac-D genes was lower than that of wild-type.These results indicated that the TaNRX1 interaction protein genes TaPDI-A,TaTRXh-A and TaPP2Ac-D enhanced the drought resistance of Arabidopsis thaliana.This study can provide candidate genes and theoretical basis for wheat drought resistance breeding.
作者
张云瑞
魏凡
杨燕宁
田淑媛
张双星
张晓科
ZHANG Yunrui;WEI Fan;YANG Yanning;TIAN Shuyuan;ZHANG Shuangxing;ZHANG Xiaoke(College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2022年第5期521-528,共8页
Journal of Triticeae Crops
基金
国家自然科学基金项目(31671693)
国家重点研发计划项目(2016YFD0101802)
陕西省重点研发计划项目(2019ZDLNY04-05)。
