摘要
目的:探讨黑色素瘤相关抗原A家族(MAGE-As)在乳腺癌患者外周血及组织中的表达,并分析其与乳腺癌患者临床病理学指标及预后的关系。方法:采用多重巢式PCR法检测106例乳腺癌患者和30例健康志愿者外周血中MAGE-As m RNA的表达水平,并利用限制性内切酶酶切法,鉴定该家族成员MAGE-A1、A2、A3、A4和A6的表达情况。采用免疫组织化学法检测了106例乳腺癌组织蜡块中MAGE-As蛋白的表达情况,同时分析外周血中MAGE-As mRNA表达与其相应肿瘤组织中MAGE-As蛋白的表达的相关性。结果:MAGE-As mRNA在乳腺癌患者外周血中阳性表达率为40.5%(43/106)。MAGE-A1、A2、A3、A4和A6的表达率分别为10.3%(11/106)、19.8%(21/106)、15.1%(16/106)、13.2%(14/106)和5.7%(6/106),MAGE-As表达率的顺序为A2>A3>A4>A1>A6。MAGE-As mRNA阳性表达与患者年龄、肿瘤大小、临床分期、淋巴结转移相关(P<0.05)。乳腺癌患者外周血中MAGE-As mRNA表达水平与其相应肿瘤组织中蛋白水平的表达呈正相关(r=0.368,P<0.01)。MAGE-As各亚型(MAGE-A1、A2、A3、A4和A6)阳性表达率与乳腺癌患者5年生存率相关(P<0.01);MAGE-As表达及淋巴结转移可作为乳腺癌患者5年生存率的独立预后影响因素(均P<0.01)。结论:MAGE-As可能作为检测乳腺癌患者外周血中循环肿瘤细胞的特异性生物标志物,其表达与乳腺癌患者预后相关。乳腺癌患者外周血中MAGE-As检测可作为监测乳腺癌预后的重要指标。
OBJECTIVE:To investigate expressions of melanoma-associated antigen A family(MAGE-As)in peripheral blood and cancer tissues from breast cancer patients,and to analyze their relationships with clinical parameters.METHODS:Expression levels of MAGE-As mRNA in peripheral blood of 106 breast cancer patients and 30 healthy controls were detected by multi-nested PCR,and expression levels of MAGE-A1,A2,A3,A4 and A6 were identified by restriction endonuclease digestion method.Expressions of MAGE-As protein in paraffin-embedded tissues of cancer tissues were detected by immunohistochemistry.Correlations between expressions of MAGE-As mRNA in peripheral blood and of MAGE-As protein in corresponding tumor tissues were analyzed.RESULTS:Positive expression rate of MAGE-As mRNA in the blood samples from patients was 40.5%(43/106).Expression rates of MAGE-A1,A2,A3,A4 and A6 were 10.3%(11/106),19.8%(21/106),15.1%(16/106),13.2%(14/106)and 5.7%(6/106),respectively.The sequence of MAGE-As gene expressions was A2>A3>A4>A1>A6.The MAGE-As mRNA expression was positively correlated with age,tumor size,clinical stage and lymph node metastasis(P<0.05).Expressions of MAGE-As mRNA in blood samples of patients were positively correlated with expression of MAGE-As protein in corresponding tumor tissues(r=0.368,P<0.01).Positive expressions of MAGE-As subtypes(MAGE-A1,A2,A3,A4 and A6)were correlated with 5-year survival rates of breast cancer patients(P<0.01).MAGE-As expression and lymph node metastasis were independent prognostic factors for 5-year survival of these patients(all P<0.01).CONCLUSION:MAGE-As may be a specific biomarker for detection of circulating tumor cells in peripheral blood of breast cancer patients,and its expression was correlated with the prognosis of these patients.MAGE-As in peripheral blood of breast cancer patients may be useful as an important biomarker to monitor prognosis of breast cancer.
作者
范晓杰
李清靖
谷丽娜
桑梅香
单保恩
FAN Xiaojie;LI Qingjing;GU Lina;SANG Meixiang;SHAN Baoen(Department of Pathology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011;Department of Clinical Laboratory,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011;Scientific Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)
出处
《癌变·畸变·突变》
CAS
2022年第3期184-191,共8页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
河北省医学科学研究重点课题资助项目(20190718)。
