摘要
本文以葡萄糖和羧甲基壳聚糖为原料,采用葡萄糖氧化酶原位交联快速制备了葡萄糖/羧甲基壳聚糖水凝胶。利用倒置法测定了水凝胶的凝胶时间和临界凝胶浓度,采用红外光谱仪和扫描电镜表征了水凝胶交联前后的变化,利用差示扫描量热仪(DSC)研究了凝胶转融温度,评估了水凝胶的溶胀性能、孔隙率、流变学性质、压缩性能和细胞毒性。研究结果表明,静电作用下,羧甲基壳聚糖与葡萄糖氧化中间产物在450 s快速形成水凝胶,凝胶内部为三维不规则多孔结构;溶胀率随着羧甲基壳聚糖含量的增加先增后减,最大接近300%;而机械强度随着组分羧甲基壳聚糖含量的增加而增加。采用Cell Counting Kit-8(CCK-8)法初步评价了水凝胶的体外细胞毒性,结果证明水凝胶具有良好的生物相容性。本研究提供了一种新的酶交联原位快速制备天然多糖水凝胶方法,并探讨了葡萄糖/羧甲基壳聚糖水凝胶的成胶原理。该方法可广泛用于制备纤维蛋白、明胶等天然聚合物水凝胶,制备的水凝胶在创伤敷料和药物释放领域极具应用潜力。
In this paper,carboxymethyl chitosan and glucose were used as raw materials and in-situ cross-linking to form a hydrogel quickly by adding glucose oxidase.The gel time,jointly with critical gel concentration were measured by inversion method.The changes before/after crosslinking of the hydrogel were characterized by infrared spectroscopy and scanning electron microscopy.The gel transition temperature was studied using a differential scanning calorimetry(DSC).The swelling properties,porosity,rheological properties,compression properties as well as cytotoxicity of the hydrogel were evaluated.It was found that,carboxymethyl chitosan and oxidation intermediate products of glucose could quickly form a hydrogel within 450 s by electrostatic interaction.The prepared hydrogels exhibited three-dimensional irregular porous structures.The swelling ratio increased first and then decreased with the increasing of carboxymethyl chitosan content,the maximum value was close to 300%.The mechanical properties increased along with the carboxymethyl chitosan content.The Cell Counting Kit-8(CCK-8)method was used to evaluate the in vitro cytotoxicity of the hydrogel,and the results showed that the hydrogel exhibited good biocompatibility.This study provided a new method for rapid preparation of natural polysaccharide hydrogels in situ by enzymatic cross-linking,and discusses the gel forming principle of glucose/carboxymethyl chitosan hydrogels.This strategy could be widely used to prepare natural polymer hydrogels such as fibrin and gelatin,and the prepared hydrogels had great application potential in wound dressings and drug release fields.
作者
裴大婷
曾志文
周小雁
国翠平
耿志杰
鲁道欢
于珊
PEI Da-ting;ZENG Zhi-wen;ZHOU Xiao-yan;GUO Cui-ping;GENG Zhi-jie;LU Dao-huan;YU Shan(Institute of Biological and Medical Engineering,Guangdong Academy of Sciences,National Engineering Research Center for Healthcare Devices,Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products,Guangzhou 510500,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2022年第5期1061-1069,共9页
Chemical Research and Application
基金
广东省科学院建设国内一流研究机构行动专项资金项目(2019GDASYL-0501003)资助
广东省基础与应用基础研究基金项目(2019A1515110207)资助
广东省省级科技计划项目(2018B090904004)资助。
