摘要
以当年生三倍体漆树幼嫩茎段为外植体,通过植物组织培养方法,找出三倍体漆树幼嫩茎段最适宜的消毒时间、方法以及最佳腋芽诱导培养基,建立三倍体漆树组织培养无菌体系。结果表明,三倍体漆树幼嫩茎段消毒最佳时间和方法为75%乙醇消毒60 s+0.1%升汞消毒4 min,腋芽诱导最佳培养基为MS+6-BA 0.3 mg/L+ZT 0.2 mg/L+活性炭0.3 g/L。
The optimal disinfection time, method and the optimal axillary bud induction medium were found by using the current year young stem segments of triploid Toxicodendron vernicifluum as explants, and the aseptic system of triploid Toxicodendron vernicifluum tissue culture was established. The results showed that the best disinfection time and method for triploid Toxicodendron vernicifluum young stem segment were 75% ethanol 60 s +0.1% mercury 4 min. The optimal medium for triploid Toxicodendron vernicifluum axillary bud induction was MS +6-BA 0.3 mg/L +ZT 0.2 mg/L +active carbon 0.3 g/L.
作者
王壮
卢宇
马东晓
WANG Zhuang;LU Yu;MA Dongxiao(Yunnan Academy of Biodiversity,Southwest Forestry University,Kunming Yunnan 650224;Key Laboratory for Forest Genetic and Tree Improvement&Propagation in Universities of Yunnan Province,Southwest Forestry University,Kunming Yunnan 650224)
出处
《现代农业科技》
2022年第9期100-102,111,共4页
Modern Agricultural Science and Technology
基金
云南省教育厅重大专项项目(ZD2013014)
云南省教育厅研究生基金项目(2016YJS100)
云南省林学一流学科建设项目(51600625)
西南林业大学大学生创新基金项目(C16011,C16013)。
关键词
三倍体漆树
无菌体系
外植体
腋芽诱导
triploid Toxicodendron vernicifluum
sterile system
explant
axillary bud induction
