摘要
为了阐明Spd(亚精胺)调控小白菜的耐盐生理机制,选用小白菜为试材,通过营养液培养试验探究叶面喷施0.4、0.8、1.2、1.6、2.0 mmol·L^(-1) Spd溶液对100 mmol·L^(-1)NaCl模拟盐胁迫条件下小白菜幼苗生长、AsA-GSH(抗坏血酸-谷胱甘肽)循环及氮代谢的影响。结果表明,NaCl胁迫显著抑制小白菜幼苗的生长以及AsA-GSH循环、氮代谢的正常运转。相比于其他浓度Spd,1.2 mmol·L^(-1) Spd对小白菜幼苗NaCl胁迫的缓解效果最佳,能明显降低MDA含量,显著增加幼苗的株高、根长、单株鲜质量和干质量;能显著提升AsA-GSH循环中抗氧化剂(AsA、GSH)含量和APX等关键酶活性,AsA/DHA和GSH/GSSG比值较NT处理分别显著增加了15.93%和6.08%;能显著提升氮代谢关键酶活性,NR、NIR、GS、GOGAT和GDH活性较NT处理分别显著增加了57.09%、28.47%、127.01%、28.79%和37.56%,促进NO_(3)^(-)-N的吸收转化。综上所述,喷施适宜浓度的Spd(1.2 mmol·L^(-1))能通过增强AsA-GSH循环及氮代谢来提升小白菜幼苗的耐盐性,有效减轻NaCl胁迫对幼苗的氧化伤害,从而促进植株生长。
In order to clarify the salt-tolerant physiological mechanism by Spd-regulated of Brassica chinensis L.,a nutrient solution culture experiment was conducted to explore the effects of foliar-spraying different concentrations of spermidine(Spd)solutions(0.4,0.8,1.2,1.6 and 2.0 mmol·L^(-1))on the growth,AsA-GSH cycle and nitrogen metabolism of Brassica chinensis L.cultivar Shanghaiqing under 100 mmol·L^(-1) NaCl simulated salt stress to clarify the salt tolerant physiological mechanism regulated by Spd.The results showed that NaCl stress obviously inhibited the growth,AsA-GSH cycle and nitrogen metabolism of Brassica chinensis L.seedlings.Compared with other concentrations of Spd,1.2 mmol·L^(-1) Spd had a better alleviation effect on NaCl stress,significantly reduced the contents of MDA and increased the plant height,root length,fresh weight and dry weight of the seedlinh.Spd at 1.2 mmol·L^(-1) significantly promoted the contents of antioxidants(AsA,GSH),the key enzyme activities such as APX in AsA-GSH cycle and the ratios of AsA/DHA and GSH/GSSG by 15.93%and 6.08%,compared to NT treatment.Moreover,1.2 mmol·L^(-1) Spd significantly improved the activities of NR,NIR,GS,GOGAT and GDH in nitrogen metabolism by 57.09%,28.47%,127.01%,28.79%and37.56%,respectively,compared to NT treatment and thus to promote the absorption and transformation of NO_(3)^(-)-N.In summary,foliar-spraying appropriate concentration of Spd(1.2 mmol·L^(-1))can improve the salt tolerance of Brassica chinensis L.seedlings by promoting the AsA-GSH cycle,nitrogen metabolism and reducing the oxidative damage,thereby increasing plant growth under NaCl stress.
作者
赵肖琼
梁泰帅
张恒慧
ZHAO Xiaoqiong;LIANG Taishuai;ZHANG Henghui(Department of Environmental and Safety Engineering,Taiyuan Institute of Technology,Taiyuan 030008,Shanxi,China;College of Pharmacy,Shanxi Medical University,Taiyuan 030001,Shanxi,China)
出处
《中国瓜菜》
CAS
北大核心
2022年第4期51-55,共5页
China Cucurbits And Vegetables
基金
山西省应用基础研究计划资助项目(201801D221279)
2021年太原工业学院院级教学改革研究项目(JG202119)。
