摘要
为研究合生元制剂对小鼠抗氧化能力、细胞因子和肠道菌群的影响,随机将48只BALB/c小鼠分为对照组、低剂量组(L)、中剂量组(M)和高剂量组(H),并灌胃生理盐水和不同剂量合生元制剂,14 d后收集小鼠粪便进行菌落计数和16S rDNA高通量测序,测定小鼠肝脏、小肠组织中抗氧化指标,检测血清中细胞因子白介素-6(interleukin-6,IL-6)、白介素-1β(interleukin-1β,IL-1β)水平。结果表明:小肠和肝脏组织中,各个剂量组总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、谷胱甘肽(glutathione,GSH)含量上升,丙二醛(malondialdehyde,MDA)含量下降。H组IL-1β水平极显著降低(p<0.05)。灌胃14 d后,各剂量组与灌胃前相比,乳杆菌和双歧杆菌数量增加,16S rDNA测序表明给予合生元制剂可以增加Rikenella、Alistipes、Bifidobacterium和Lactobacillus等丰富度。灌胃上述合生元制剂可以提高小鼠抗氧化能力,降低细胞因子水平,促进乳杆菌和双歧杆菌繁殖,对肠道菌群具有调节作用。
This study aimed to test the effects of synbiotics on the antioxidant capacity and intestinal flora of mice.Forty-eight male BALB/c mice were randomly assigned into the control(normal saline)group and lowdose(L),medium-dose(M),and high-dose(H)synbiotic groups and administrated with corresponding agents by gavage.After 14 days,the feces were collected for the characterization of intestinal flora changes by colony counting and 16S rDNA high-throughput sequencing.The antioxidant indexes in the liver and small intestine tissues and the levels of interleukin-6(IL-6)and interleukin-1β(IL-1β)in the serum were measured.In the small intestine and liver tissues,the activities of total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px),and glutathione(glutathione,GSH)rose,and the malondialdehyde(MDA)content reduced after the administration of synbiotics.The level of IL-6 in group H declined(p<0.01).The number of lactobacilli and bifidobacteria in each synbiotics group increased after 14 days of administration.The 16S rDNA high-throughput sequencing results demonstrated that the administration of synbiotics increased the abundance of Rikenella,Alistipes,Bifidobacterium and Lactobacillus.The administration of the above-mentioned synbiotics could improve the antioxidant capacity,reduce the levels of inflammatory cytokines,increase the number of lactobacillus and bifidobacteriain the intestine,and regulate the intestinal flora.
作者
王涵
刘倩
郭子晨
王韵婷
王伶毓
赵江燕
张连中
孙雅煊
WANG Han;LIU Qian;GUO Zi-chen;WANG Yun-ting;WANG Ling-yu;ZHAO Jiang-yan;ZHANG Lian-zhong;SUN Ya-xuan(College of Biochemical Engineering,Beijing Union University,Beijing 100023,China;Beijing Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191,China;Beijing Hongce Technology Development Co.,Ltd.,Beijing 100176,China)
出处
《食品研究与开发》
CAS
北大核心
2022年第7期1-8,共8页
Food Research and Development
基金
国家自然科学基金(11975048)
北京联合大学开放课题(SWHX202103)
北京联合大学科研项目(XP202007)。
关键词
益生菌
肠道菌群
细胞因子
抗氧化
16S
r
DNA高通量测序
probiotic
intestinal flora
cytokines
antioxidant capacity
16S rDNA high throughput sequencing
