摘要
【目的】探讨“痰邪”在宫腔粘连(intrauterine adhesions,IUA)中医发病机制中的作用。【方法】收集60例IUA患者,总结其中医证候特点和宫腔镜下表现,分析其中医病机;取30例典型IUA患者粘连组织,免疫组化法检测黏附相关分子转化生长因子β1(TGF-β1)及其下游蛋白Smad2/3表达,并与20例非IUA患者(正常对照组)的子宫内膜组织作对照。【结果】(1)以传统外在证候辨证,IUA可辨为肾虚、肾虚血瘀等证;结合宫腔镜下表现,认为“痰凝”为IUA患者主要证型。(2)IUA患者粘连组织中TGF-β1与Smad2/3表达升高,阳性率分别为86.7%(26/30)、90.0%(27/30),明显高于正常子宫内膜组织的5.0%(1/20)和15.0%(3/20),差异均有统计学意义(P<0.05)。【结论】“痰邪”在IUA中医发病机制中起关键作用,局部黏附相关分子高表达为其微观机制。
Objective To investigate the role of phlegm pathogen in traditional Chinese medicine(TCM)pathogenesis of intrauterine adhesions(IUA).Methods Sixty patients with IUA were collected,TCM syndromes and hysteroscopic manifestations of the IUA patients were summarized,and their TCM pathogenesis was analyzed.Adhesive tissues were sampled from 30 typical IUA patients for the detection of the expression of the adhesionassociated molecule of transforming growth factorβ1(TGF-β1)and its downstream protein Smad2/3 by immunohistochemistry.The findings were compared to the endometrial tissues from 20 non-IUA patients(normal control group).Results(1)Following the traditional syndrome differentiation,IUA can be differentiated as kidney deficiency syndrome and kidney deficiency with blood stasis syndrome.When taking the hysteroscopic findings into account,IUA can be mainly identified as the phlegm accumulation syndrome.(2)The expression levels of TGF-β1and Smad2/3 in the adhesive tissues of IUA patients were increased,and the positive rate was 86.7%(26/30)and90.0%(27/30),respectively,which was significantly higher than that of the normal endometrial tissues[5.0%(1/20)and 15.0%(3/20),respectively],the difference being statistically significant(P<0.05).Conclusion Phlegm pathogen plays a key role in the TCM pathogenesis of IUA,and the high expression of local adhesionrelated molecules contributes to its microscopic mechanism.
作者
侯建峰
杜娟
黎丽娜
李小丹
钟文珍
张潇涵
吴贻平
HOU Jian-Feng;DU Juan;LI Li-Na;LI Xiao-Dan;ZHONG Wen-Zhen;ZHANG Xiao-Han;WU Yi-Ping(Zhuhai Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine,Zhuhai 519000 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2022年第4期746-749,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
珠海市医疗卫生科技计划项目(编号:20171009E030068)。
关键词
宫腔粘连
痰邪
中医发病机制
TGF-Β1
SMAD2/3
intrauterine adhesions(IUA)
phlegm pathogen
the traditional Chinese medicine(TCM)pathogenesis
transforming growth factorβ1(TGF-β1)
Smad2/3
