摘要
目的制备乳粉中克罗诺杆菌属(阪崎肠杆菌)检验能力验证样品,并应用于2018—2020年度实验室能力验证考核。方法将背景菌株和克罗诺杆菌属通过生化、基质辅助激光解吸电离飞行时间质谱鉴定确认菌株种属。采用冷冻干燥技术制备的阴性样品(含背景菌)和阳性样品(含背景菌和克罗诺杆菌属)。随机抽取阳性样品和阴性样品各20份进行均匀性检验,然后将样品分别存放于-20℃和4℃进行储藏稳定性检验,存放于25℃和37℃进行运输稳定性检验。向参加考核的实验室发放样品并提供作业指导书,回收各实验室结果进行统计。结果菌株种属鉴定结果与预期结果一致,均匀性、储藏稳定性和运输稳定性均满足要求。2018—2020年度考核结果满意率分别为97.1%、94.7%和100%。结论制备的样品满足此能力验证项目的需求,通过组织能力验证考核可以反映出我国实验室之间差异,有助于进一步提高实验室检验水平。
Objective To prepare proficiency testing samples of Cronobacter spp.and apply to laboratory test in 2018-2020.Methods The background bacteria and Cronobacter spp.were identified by biochemical reaction and MALDI-TOF MS.Cronobacter spp.negative samples(background bacteria only)and positive samples(background bacteria and Cronobacter spp.)were prepared by freeze-drying.20 samples were selected randomly for uniformity test.The samples were stored respectively at-20℃and 4℃to evaluate the storage stability,at 25℃and 37℃for 7 d to evaluate the transport stability.Samples were distributed to laboratories participating in the proficiency with operation instruction,and the feedback result from laboratories were analyzed.Results The identified result of all the related strains were correct.Both of positive samples and negative samples were uniform and stable.The satisfaction rates of assessment result were 97.1%,94.7%and 100%respectively in 2018-2020.Conclusion The samples could meet the requirements of proficiency test.By organization proficiency test,the difference of laboratories can be reflected which is helpful to further improve the laboratory test capability.
作者
刘娜
赵琳娜
王学硕
崔生辉
LIU Na;ZHAO Linna;WANG Xueshuo;CUI Shenghui(National Institutes for Food and Drug Control,Beijng 100050,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2022年第1期29-33,共5页
Chinese Journal of Food Hygiene
基金
科技部“食品安全关键技术研发”重点专项(2018YFC1604303)
中国食品药品检定研究院中青年基金(2020C3)。
关键词
克罗诺杆菌属
能力验证
样品研制
考核
Cronobacter spp.
proficiency test
sample preparation
assessment
