摘要
目的研究肺腺癌(lung adenocarcinoma,LA)患者血清中微核糖核酸(miRNA)在肺腺癌患者及正常人群组血清中的表达水平,分析其在肺腺癌诊断中的临床价值。方法收集60例肺腺癌及40例正常人群血清,通过实时荧光定量聚合酶链反应(quantitative real time PCR,qRT PCR)检测各组血清miR-498,miR-339-5p和miR-210-3p的表达情况。统计分析各组miRNA的表达差异以及单个miRNA在肺腺癌诊断中的价值,进一步用统计学方法分析三者联合检测的诊断价值。结果相比正常人群,肺腺癌患者血清中miR-210-3p表达增加(6.41±1.85 vs 4.52±1.45),miR-498(2.09±0.88vs 3.01±0.69)和miR-339-5p(0.8±0.53 vs 1.24±0.58)表达下降,差异有统计学意义(t=4.72,1.34,2.75,均P<0.05)。受试者工作特征曲线下面积(AUC)分析结果显示,miR-498,miR-339-5p和miR-210-3p的AUC分别为0.788(95%CI0.695~0.864),0.715(95%CI 0.616~0.801)和0.799(95%CI 0.707~0.872);三者联合检测在肺腺癌诊断中AUC值为0.902(95%CI 0.826~0.952),三者联合检测优于单个miRNA的检测,差异有统计学意义(t=14.09~18.65,均P<0.05)。结论miR-498,miR-339-5p和miR-210-3p在肺腺癌患者血清中的表达情况改变,对肺腺癌具有一定的临床诊断价值。
Objective The expression level of micro ribonucleic acid(miRNA)in serum in patients with lung adenocarcinoma(LA)and in the serum of normal population groups was analyzed,and its clinical value in the diagnosis of lung adenocarcinoma was analyzed.Methods Serums from 60 cases of LA and 40 normal physical examination subjects were collected,and the expressions of miR-498,miR-339-5p and miR-210-3p in each groups were detected by real time fuorescence quantitative PCR.The expression differences of miRNA in each group and the value of single serum miRNA in the diagnosis of lung adenocarcinoma were statistically analyzed,and the diagnostic value of three miRNA combined detection was further analyzed by statistical methods.Results Compared with the normal population,the expression of miR-210-3p(6.41±1.85vs 4.52±1.45)was increased in the serum of patients with lung adenocarcinoma,and the expressions of miR-498(2.09±0.88vs 3.01±0.69)and miR-339-5p(0.8±0.53 vs 1.24±0.58)were decreased,and the differences were statistically significant(t=4.72,1.34,2.75,all P<0.05).Area under the receiver operating characteristic curve(AUC)analysis results showed that the AUC of miR-498,miR-339-5p and miR-210-3p was 0.788(95%CI 0.695~0.864),0.715(95%CI 0.616~0.801)and 0.799(95%CI 0.707~0.872),respectively.In the diagnosis of lung adenocarcinoma,the three combined was 0.902(95%CI 0.826~0.952),which was better than any of three miRNAs(χ^(2)=14.09~18.65,all P<0.05).Conclution The changes in the expression of miR 498,miR 3395p and miR 2103p in the serum of patients with LA can be used as a certain clinical value in the diagnosis of lung adenocarcinoma.
作者
董胜鉴
黄丽丽
李树锦
DONG Sheng-jian;HUANG Li-li;LI Shu-jin(Department of Clinical Laboratory,the Second People’s Hospital of Hefei City,Hefei 230001,China)
出处
《现代检验医学杂志》
CAS
2022年第2期58-60,共3页
Journal of Modern Laboratory Medicine
