摘要
目的 采用基于增强型脂质去除吸附剂(enhanced matrix removal lipid, EMR-lipid)的QuEChERS净化技术,建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)同时检测食用植物油中叔丁基对羟基茴香醚(butylhydroxyanisole,BHA)、叔丁基对苯二酚(tert-butyl hydroquinone, TBHQ)、2,4,5-三羟基苯丁酮(2,4,5-trihydroxybutyrophenone, THBP)、没食子酸丙酯(propyl gallate, PG)、2,6-二叔丁基-4-羟甲基苯酚(2,6-di-tert-butyl-4-hydroxymethylphenol, Ionox-100) 5种合成抗氧化剂的检测方法。方法 样品经乙腈提取和EMR-Lipid除脂净化, C_(18)色谱柱分离,采用乙腈和水作为流动相梯度洗脱,质谱电喷雾和多反应监测模式(multiple reaction monitoring,MRM)进行检测分析。结果 5种抗氧化剂在0.05-5.00μg/mL范围内线性关系良好(r≥0.993),检出限为0.01-0.08 mg/kg。在0.2、1.0、5.0 mg/kg 3个不同浓度加标水平的平均回收率为82.5%-103.6%,相对标准偏差为1.7%-6.8%(n=6)。结论 本方法前处理简单快速、净化效果好,适用于植物油中BHA、TBHQ、THBP、PG和Ionox-100合成抗氧化剂的检测。
Objective To establish a method for the determination of 5 kinds of synthetic antioxidants including butyl hydroxyanisole(BHA), tert-butyl hydroquinone(TBHQ), 2,4,5-trihydroxybutyrophenone(THBP), propyl gallate(PG), 2,6-di-tert-butyl-4-hydroxymethylphenol(Ionox-100) by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with QuEChERS purification technology based on enhanced matrix removal lipid(EMR-lipid). Methods Samples were extracted by acetonitrile, purified by EMR-lipid, separated on a C_(18)column by gradient elution with acetonitrile-water as mobile phase, and detected by multiple reaction monitoring mode(MRM)with electrospray ionization. Results Five kinds of antioxidants had good linear relationships(r≥0.993) in the range of 0.05-5.00 μg/mL, the limits of detection were 0.01-0.08 mg/kg. The average recoveries at 3 spiked levels of 0.2, 1.0 and 5.0 mg/kg were 82.5%-103.6%, and the relative standard deviations were 1.7%-6.8%(n=6). Conclusions This method is simple, rapid and has good purification effect, which is suitable for the determination of BHA、TBHQ、THBP、PG and Ionox-100 in vegetable oils.
作者
王凤丽
王东旭
王新财
杨帆
王艳艳
冯秋芳
WANG Feng-Li;WANG Dong-Xu;WANG Xin-Cai;YANG Fan;WANG Yan-Yan;FENG Qiu-Fang(Huzhou Institute for Food and Drug Control,Huzhou 313000,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2022年第4期1164-1169,共6页
Journal of Food Safety and Quality
基金
浙江省市场监督管理局科研计划项目(20200124)。
