摘要
外泌体是由生物体内各种细胞主动分泌的脂质双分子层膜性囊泡,似纳米粒,具有信使靶向性,与中医药超分子“气析”理论结合可构建中医药现代化实验研究思路与策略。外泌体由各细胞分泌,膜内含核酸、蛋白质、脂质及小分子代谢物质等,亲本能精准协调各细胞间的功能,浓缩传递亲本细胞的功能信息,是体现细胞功能的简洁形式;同时荷载了中医药超分子“气析”理论的“印迹模板”,若对其包裹的基因-蛋白质-脂质-小分子间的“印迹模板”运载规律进行研究,可建立起中医药理论现代化实验研究思路和策略,揭示机体经络脏腑功能。笔者首先对外泌体的现状,包括发现、分泌、特点、功能、归属、摄取、研究方法、应用现状进行综述,阐述其精准信使靶向性的运载工具天然属性,体现出了经络脏腑微观物质的运行规律;其次,探讨其作为中医药超分子“气析”理论的“印迹模板”重要载体,融合中医药超分子化学研究方法提出中医药经络脏腑与归经的微观物质基础、中药复方配伍、中药靶向制剂靶向性的实验研究思路与策略。
Exosomes are lipid bilayer membranous vesicles actively secreted by various cells in the organism,which are like nanoparticles and have messenger targeting.Combining with the theory of supramolecular"Qi chromatography"of traditional Chinese medicine(TCM),research ideas and strategies of modernization of TCM can be constructed.Exosomes are secreted by cells,and the membrane contains nucleic acids,proteins,lipids and small molecular metabolites and others,which can accurately coordinate the functions of each cell,concentrate and transmit the functional information of the parent cell,and is the concise form of reflecting cell functions.At the same time,it is loaded with the"imprinted templates"of the supramolecular"Qi chromatography"theory of TCM.If the"imprinted templates"carrying rules among the geneprotein-lipid-small molecules wrapped in it is studied,the modern experimental research ideas and strategies of TCM theory can be established for revealing the functions of the body's meridians and viscera.Firstly,the present situation of exosomes,including discovery,secretion,characteristics,functions,attribution,uptake,research methods and application status,were reviewed in this paper.And the natural properties of its precise messenger targeted delivery vehicle were elaborated,reflecting the operation law of microscopic substances in meridians and viscera.Secondly,to explore it as an important carrier of the concentrated"imprinted templates"of the supramolecular"Qi chromatography"theory of TCM,and integrating the research methods of exosomes and supramolecular chemistry of TCM,this paper proposes experimental research ideas and strategies on the microscopic material basis of meridians and viscera,compatibility of TCM compound,and targeting of TCM targeted preparations.
作者
陈定芳
吴月峰
李海英
邓凯文
孟蕾
王韧
肖美凤
周逸群
潘雪
贺福元
CHEN Ding-fang;WU Yue-feng;LI Hai-ying;DENG Kai-wen;MENG Lei;WANG Ren;XIAO Mei-feng;ZHOU Yi-qun;PAN Xue;HE Fu-yuan(School of Pharmacy,Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine,Supramolecular Mechanism and Mathematic-physics Chracterization for Chinese Materia Medica,Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2022年第7期198-206,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81703824,81803729,81903759,81874507)
湖南省科技厅重点实验室建设项目(2016TP1017)
湖南省自然科学基金项目(2019JJ50430)
湖南省教育厅创新平台开放基金项目(18K071)
湖南省“国内一流培育学科”药学2018年开放基金项目(2018YX06)
湖南中医药大学科研基金项目(2019XJJJ024)
湖南中医药大学药学一流学科基金项目(2021YX15)。
关键词
外泌体
中医药理论
印迹模板
超分子“气析”理论
靶向性
靶向制剂
方剂配伍
exosomes
theory of traditional Chinese medicine
imprinting templates
supramolecular"Qi chromatography"theory
targeting
targeted preparations
formula compatibility
