摘要
目的探讨子痫前期(PE)患者胎盘组织中微RNA(miR)-210、Foxp3的表达及临床意义。方法选择2018年6月至2019年12月南阳市中心医院收治的且以剖宫产方式分娩的36例PE患者为PE组,选择同期正常妊娠孕妇35例为正常妊娠组,另选择体检健康的未妊娠女性40例为未妊娠组。采用流式细胞术检测3组受试者外周静脉血CD4^(+)CD25^(+)CD127^(-)调节性T细胞(Treg细胞)、CD4^(+)辅助性T淋巴细胞(Th细胞)水平,计算CD4^(+)CD25^(+)CD127^(-)Treg细胞与CD4^(+)Th细胞的比值(CD4^(+)CD25^(+)CD127^(-)/CD4^(+));采用酶联免疫吸附试验检测3组受试者血清白细胞介素(IL)-6、IL-10、IL-17水平;实时荧光定量聚合酶链反应法检测PE组和正常妊娠组受试者胎盘组织中Foxp3 mRNA、miR-210表达,Western blot法检测胎盘组织中Foxp3蛋白表达。结果PE组、正常妊娠组与未妊娠组受试者外周静脉血CD4^(+)CD25^(+)CD127^(-)/CD4^(+)分别为(6.72±0.31)%、(8.92±0.37)%、(8.85±0.34)%;PE组受试者外周静脉血CD4^(+)CD25^(+)CD127^(-)/CD4^(+)显著低于正常妊娠组和未妊娠组(t=27.136、28.581,P<0.05);正常妊娠组与未妊娠组受试者外周静脉血CD4^(+)CD25^(+)CD127^(-)/CD4^(+)比较差异无统计学意义(t=0.849,P>0.05)。3组受试者血清IL-6、IL-10、IL-17水平比较差异有统计学意义(F=53.584、133.925、42.392,P<0.05)。PE组受试者血清IL-6水平显著高于正常妊娠组(t=9.170,P<0.05),正常妊娠组受试者血清IL-6水平显著低于未妊娠组(t=8.844,P<0.05);PE组与未妊娠组受试者血清IL-6水平比较差异无统计学意义(t=1.608,P>0.05)。PE组受试者血清IL-10水平显著低于正常妊娠组和未妊娠组(t=14.929、4.892,P<0.05),正常妊娠组受试者血清IL-10水平显著高于未妊娠组(t=10.914,P<0.05)。PE组受试者血清IL-17水平显著高于正常妊娠组和未妊娠组(t=8.184、3.038,P<0.05),正常妊娠组受试者血清IL-17水平显著低于未妊娠组(t=7.325,P<0.05)。PE组受试者胎盘组织中Foxp3 mRNA相对表达量显著低于正常妊娠组(t=13.948,P<0.05),miR-210相对表达量显著高于正常妊娠组(t=16.565,P<0.05)。PE组和正常妊娠组受试者胎盘组织中Foxp3蛋白相对表达量分别为0.97±0.11、1.38±0.18,PE组受试者胎盘组织中Foxp3蛋白相对表达量显著低于正常妊娠组(t=11.625,P<0.05)。结论PE患者外周静脉血Treg细胞水平显著降低,Treg/Th17细胞失衡,可破坏母体对胎儿的免疫耐受;PE患者胎盘组织中Foxp3表达水平下降可能与miR-210表达上调有关。
Objective To investigate the expression and clinical significance of microRNA(miR)-210 and Foxp3 in placental tissues of patients with preeclampsia(PE).Methods Thirty-six PE patients who underwent cesarean section in Nanyang Central Hospital from June 2018 to December 2019 were selected as the PE group,thirty-five normal pregnancy pregnant women during the same period were selected as the normal pregnancy group,and 40 healthy non-pregnant women were selected as the non-pregnant group.The levels of CD4^(+)CD25^(+)CD127^(-)regulatory T cells(Treg cell)and CD4^(+)helper T lymphocytes(Th cell)in peripheral venous blood of the subjects in the three groups were detected by flow cytometry,and the ratio of CD4^(+)CD25^(+)CD127^(-)Treg cells to CD4^(+)Th cells(CD4^(+)CD25^(+)CD127^(-)/CD4^(+))was calculated.The levels of serum interleukin(IL)-6,IL-10 and IL-17 of the subjects in the three groups were detected by enzyme-linked immunosorbent assay.The expressions of Foxp3 mRNA and miR-210 in placental tissues of the subjects in the PE group and normal pregnancy group were detected by real-time fluorescence quantitative polymerase chain reaction,and the expression of Foxp3 protein in placental tissues was detected by Western blot.Results The CD4^(+)CD25^(+)CD127^(-)/CD4^(+)in peripheral venous blood of subjects in the PE group,normal pregnancy group and non-pregnancy group was(6.72±0.31)%,(8.92±0.37)%and(8.85±0.34)%,respectively.The CD4^(+)CD25^(+)CD127^(-)/CD4^(+)in peripheral venous blood of subjects in the PE group was significantly lower than that in the normal pregnancy group and non-pregnancy group(t=27.136,28.581;P<0.05).There was no significant difference in the CD4^(+)CD25^(+)CD127^(-)/CD4^(+)in peripheral venous blood of subjects between the normal pregnancy group and non-pregnancy group(t=0.849,P>0.05).There was significant difference in the levels of serum IL-6,IL-10 and IL-17 among the three groups(F=53.584,133.925,42.392;P<0.05).The level of serum IL-6 in the PE group was significantly higher than that in the normal pregnancy group(t=9.170,P<0.05),and the level of serum IL-6 in the normal pregnancy group was significantly lower than that in the non-pregnancy group(t=8.844,P<0.05),but there was no significant difference in the level of serum IL-6 between the PE group and non-pregnant group(t=1.608,P>0.05).The level of serum IL-10 in the PE group was significantly lower than that in the normal pregnancy group and non-pregnancy group(t=14.929,4.892;P<0.05),and the level of serum IL-10 in the normal pregnancy group was significantly higher than that in the non-pregnancy group(t=10.914,P<0.05).The level of serum IL-17 in the PE group was significantly higher than that in the normal pregnancy group and non-pregnancy group(t=8.184,3.038;P<0.05),and the level of serum IL-17 in the normal pregnancy group was significantly lower than that in the non-pregnancy group(t=7.325,P<0.05).The relative expression of Foxp3 mRNA in placental tissues of subjects in the PE group was significantly lower than that in the normal pregnancy group(t=13.948,P<0.05),and the relative expression of miR-210 in placental tissues of subjects in the PE group was significantly higher than that in the normal pregnancy group(t=16.565,P<0.05).The relative expression of Foxp3 protein in placental tissues of subjects in the PE group and normal pregnancy group was 0.97±0.11 and 1.38±0.18,respectively.The relative expression of Foxp3 protein in placental tissues of subjects in the PE group was significantly lower than that in the normal pregnancy group(t=11.625,P<0.05).Conclusion The level of Treg cells in peripheral venous blood of PE patients is significantly decreased,and the Treg/Th17 cell is unbalanced,which can destroy maternal immune tolerance to the fetus.The decrease of Foxp3 expression in placental tissues of PE patients may be related to the up-regulation of miR-210 expression.
作者
张澍
王春延
忽平
姚利
ZHANG Shu;WANG Chunyan;HU Ping;YAO Li(Department of Gynaecology and Obstetrics,Nanyang Central Hospital/Nanyang Central Hospital Affiliated to Zhengzhou University,Nanyang 473000,Henan Province,China)
出处
《新乡医学院学报》
CAS
2022年第2期165-169,共5页
Journal of Xinxiang Medical University
