摘要
[目的]为了鉴定甘肃武威地区某牛场中牛病毒性腹泻病的发病情况,分别采集了13只病牛的血液样品、粪便样品配合诊断。[方法]通过血清学诊断方法和PCR鉴定的方法对样本进行了检测。[结果]显示血清学方法中的13份血液样本有8份为抗原检测为阳性,粪便样本提取RNA,进行RT-PCR同样可以检测到8份样本中扩增得到片段大小为267 bp的条带,检测的13份样本有8份样本的血清学检测和粪便PCR检测均为阳性。[结论]本次检测的BVDV阳性率为61.54%,结果表明本次检测的甘肃武威地区某养殖场的牛病毒性腹泻抗原阳性率较高,需要加强牛病毒性腹泻的防控,采取科学的手段治理该类疾病降低其对养牛产业的损失。
[Objective]To identify the incidence of bovine viral diarrhea in a cattle farm in Wuwei area of Gansu Province,the blood and fecal samples of 13 sick cattle were collected for diagnosis.[Method]The samples were detected by serological diagnosis and PCR identification.[Results]It showed that 8 of 13 blood samples were positive,and RNA was extracted from fecal samples,RT-PCR could also detect the 267 bp band in these 8 samples.Of the 13 samples tested,8 samples were positive in serological test and fecal PCR test.The positive rate of this test was 61.54%.[Conclusion]The results showed that the positive rate of bovine viral diarrhea in a farm in the Wuwei area of Gansu Province was relatively high,it is necessary to strengthen the prevention and control of bovine viral diarrhea,take scientific measures to control the disease,and reduce the loss of cattle industry.
作者
苏文娟
孔伟
张宝良
SU Wen-juan;KONG Wei;ZHANG Bao-liang(Gansu Livestock Breeding and Improvement Management Station, Wuwei, Gansu 733000)
出处
《中国牛业科学》
2021年第6期100-102,共3页
China Cattle Science
关键词
牛病毒性腹泻
血清学检测
PCR鉴定
bovine viral diarrhea
serological test
PCR identification
