摘要
时差成像(time-lapse imaging,TLI)技术是近年来出现的可以实时监控记录胚胎发育过程的新型无创胚胎培养及观察技术。分析TLI记录的形态动力学参数将有助于更全面地评估胚胎质量,以筛选出最优质的胚胎。通过综述性分析国内外已发表的文献,阐述了目前常用的与胚胎发育息息相关的形态动力学参数,并探讨其与胚胎发育潜能和染色体整倍性的相关性,及其在国内外应用于优选胚胎的现状,并客观评价了TLI技术目前在临床应用中的利弊。TLI可以弥补传统胚胎培养系统和形态学评分的不足,能够帮助胚胎学家更加精确地选择具有发育潜能的胚胎,是今后胚胎培养的趋势。但是作为一项新技术,TLI在应用中仍需要不断地改进和完善,尚需要更多研究来优化胚胎选择参数和建立胚胎选择模型。
Time-lapse imaging(TLI)is a novel and noninvasive embryo culture and observation technology that can monitor and record the embryonic development in real-time.The analysis of morphokinetic parameters recorded by TLI will help to evaluate the quality of embryos more comprehensively,so as to select the best embryo(s)for embryonic transfer(ET).This review expounds those commonly-used morphokinetic parameters that are closely linked with embryonic development,and discusses the association between these parameters and embryonic development potential and chromosome euploidy.The application status of TLI in embryo selection at present was evaluated,including its advantages and disadvantages.As described in the literature,TLI makes up the flaw of the traditional embryo culture system and morphologic evaluation for embryos,which is helpful for embryologists to select the embryos with developmental potential more accurately.TLI may be the trend of human embryo culture in the future.However,as a new technology,TLI still needs further improvement and perfection,and more research in the embryo selection parameters and embryonic selection models.
作者
陈志坚
汪彩珠(审校)
CHEN Zhi-jian;WANG Cai-zhu(Reproductive Medicine Center,Meternity and Child Health Care of Guangxi Zhuang Autonomous Region,Nanning 530012,China)
出处
《国际生殖健康／计划生育杂志》
CAS
2022年第2期139-142,共4页
Journal of International Reproductive Health/Family Planning
基金
广西医疗卫生适宜技术开发与推广应用项目(S2021072)。
关键词
胚胎发育
动力学
时差成像技术
胚胎评估
形态学
Embryonic development
Kinetics
Time-lapse imaging technology
Embryo evaluation
Morphology
