摘要
为探讨顺-冷杉醇合成基因NtCPS2对烟草光合作用的影响,以烤烟品系8306、8306B(纯合突变植株T;代)、新豫烟11号(MSK326×8306B)和烤烟品种MSK326、豫烟11号(MSK326×8306)为试验材料,比较其顺-冷杉醇、赤霉素、脱落酸含量及相关合成基因相对表达量,光合参数及光合作用相关基因相对表达量等差异。结果表明,8306B较8306、新豫烟11号较豫烟11号的顺-冷杉醇含量均降低,赤霉素含量分别升高7.90%、9.79%,脱落酸含量分别降低35.87%、11.60%,赤霉素合成途径中正调控关键基因GA20ox表达量显著升高,负调控关键基因GA2ox表达量显著降低,脱落酸合成途径中关键基因PSY、NCED相对表达量显著降低,气孔闭合比率分别降低43.75%、8.33%,气孔导度增大,净光合速率升高,光合作用相关酶Rubisco、GAPDH的活性及基因相对表达量升高,光合产物发生变化,同时株高、叶数、节距显著升高。NtCPS2基因不仅影响烟草二萜类代谢途径,降低顺-冷杉醇含量,增加赤霉素含量,而且影响光合作用,导致光合效率提高,增大株型。本研究结果为NtCPS2基因对植物光合作用的反馈调节提供了例证。
To investigate the effects of cis-abienol biosynthesis gene NtCPS2 on tobacco photosynthesis,we compared the differences of photosynthetic parameters,enzyme activity,products,stomatal opening,cis-abienol,GA3,ABA contents and related synthetic genes among 8306,8306 B,New Yuyan11(MSK326×8306 B),MSK326 and Yuyan11 MSK326×8306.The results showed that when compared between 8306 B and 8306,Yuyan11 and New Yuan11,the cis-abienol content of 8306 B and Yuyan 11 were lower,the GA3 content was increased by 7.90%and 9.79%,the ABA content was decreased by 35.87%and 11.60%,respectively.Furthermore,the expression level of GA20ox,a key positive regulation gene in GAs synthesis,was significantly increased,whereas the expression levels of negative regulation key gene GA2ox and ABA synthesis genes PSY and NCED were significantly decreased.In other aspects,the stomatal closure ratio was decreased by 43.75%and 8.33%,respectively.The stomatal conductance net photosynthetic rate the activities of photosynthesis-related enzymes Rubisco and GAPDH,and the relative gene expression were increased.The photosynthates were changed.Meanwhile the height,number of leaves and pitch of tobacco plant were increased significantly.These findings indicate that the NtCPS2 gene not only affected the diterpenoid metabolic pathway,reduced the content of cis-abienol and increased the content of GA3,but also affected the photosynthesis improved the photosynthetic efficiency and the plant-type.This research provides an example for the feedback regulation of terpenoids on plant photosynthesis.
作者
贺凌霄
韩文龙
李波
江智敏
徐敏
李芳芳
郑聪
徐世晓
HE Lingxiao;HAN Wenlong;LI Bo;JIANG Zhimin;XU Min;LI Fangfang;ZHENG Cong;XU Shixiao(College of Tobacco,Henan Agricultural University,Zhengzhou,Henan 450002;China Tobacco Zhejiang Industrial Co.,Ltd.,Hangzhou,Zhejiang 310008;Henan Company of China National Tobacco Corporation,Zhengzhou,Henan 450002;Pucheng Branch of Nanping Tobacco Company of Fujian Province,Nanping,Fujian 353400)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第3期554-566,共13页
Journal of Nuclear Agricultural Sciences
基金
中国烟草总公司河南公司科技重点项目(2021410000240021)
湖南烟草总公司长沙公司科技重点项目(21-23A04)
广西壮族自治区烟草总公司百色公司科技重点项目(2012145100020165)
福建省烟草公司南平市公司科技重点项目(NYK2021-09-03)。
