摘要
目的:明确芍药汤对湿热泄泻大鼠的止泻抗炎作用和肠道菌群调节作用,探讨芍药汤中"通因通用"法的作用机制。方法:采用高温+高湿+高糖高脂饮食+致病因子方式造模,分别设置正常组(不造模)、模型组(给予生理盐水),芍药汤全方组(生药5.62 g·kg^(-1)),芍药汤全方去大黄组(生药5.15 g·kg^(-1)),单味大黄组(生药0.01 g·kg^(-1)),给药5日,每天早晚2次。每天晚上给药3 h后用腹泻指数评估各给药组止泻效果,最后1次给药3 h后用酶联免疫吸附测定法检测大鼠血清中大鼠肿瘤坏死因子-α(TNF-α),大鼠白细胞介素-6(IL-6),大鼠白细胞介素-1β(IL-1β)和大鼠白细胞介素-2(IL-2)含量,用16sDNA对大鼠粪便中菌群结构进行表征。结果:与模型组比较,芍药汤全方、芍药汤去大黄和单味大黄水煎液均可显著降低大鼠腹泻指数(P<0.01),其起效速度为芍药汤全方>芍药汤去大黄>单味大黄。与模型组比较,3种给药方案均可显著降低大鼠体内炎症因子水平(P<0.01),其中芍药汤全方组效果最佳,而单味大黄组和芍药汤去大黄组次之,二者之间并无显著性差异。与模型组比较,各组给药方案均可以显著减少致病菌和条件致病菌(如Escherichia-Shigella,Prevotella,Enterorhabdus和Bacteroides)的丰度,增加益生菌(如Ruminococcus,Turicibacter和Lachnospiraceae)的增殖(P<0.01)。其中单味大黄组、芍药汤全方组和正常组的肠道菌群结构更加接近,芍药汤去大黄组较正常组和其余两个给药组差异较大(P<0.01)。结论:芍药汤可通过抗炎和调节肠道菌群紊乱两个环节促进湿热泄泻大鼠的转归,方中大黄对于降低有害菌丰度、促进益生菌增殖方面起到关键作用,是芍药汤促进肠道菌群再平衡的关键,也初步证实了芍药汤方用大黄"通因通用"治疗湿热泄泻的科学性。
Objective: To investigate the effect of Shaoyaotang on diarrhea, inflammation, and intestinal flora in rats with dampness-heat diarrhea and explore the mechanism of therapeutic principle "treating incontinent syndrome with dredging method" of Shaoyaotang. Method: The dampness-heat diarrhea model was induced by high temperature,high humidity,high sugar and fat diet,and pathogenic factors. The rats were divided into normal group,model group(normal saline),Shaoyaotang group(5.62 g·kg^(-1)),Rhei Radix et Rhizoma(RRER)-free Shaoyaotang group(5.15 g·kg^(-1)),and RRER group(0.01 g·kg^(-1)). The rats were treated correspondingly for five days,twice a day in the morning and evening. The diarrhea index was used to evaluate the antidiarrheal effect of each group three hours after the administration in the evening. The levels of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-2,and IL-6 in the serum were detected by enzyme-linked immunosorbent assay(ELISA)three hours after the last administration. The structure of intestinal flora in feces was characterized by 16 sDNA. Result: Compared with the model group,the Shaoyaotang group,the RRERfree Shaoyaotang group,and the RRER group showed reduced diarrhea index(P<0.01),with the onset rates ranking as the Shaoyaotang group>the RRER-free Shaoyaotang group>the RRER group. Those three groups with drug intervention all showed decreased levels of inflammatory factors(P<0.01),especially the Shaoyaotang group,and no significant difference was observed between the RRER group and the RRER-free Shaoyaotang group. The abundance of pathogenic bacteria and conditioned pathogens(e.g. Escherichia-Shigella,Prevotella,Enterorhabdus,and Bacteroides)was reduced and the proliferation of probiotics(such as Ruminococcus,Turicibacter,and Lachnospiraceae)was increased in the groups with drug intervention(P<0.01). For the structure of intestinal flora,the RRER group and the Shaoyaotang group were close to the normal group,and the RRER-free Shaoyaotang group was different from the other three groups(P<0.01). Conclusion: Shaoyaotang can improve the outcome of rats with dampness-heat diarrhea through anti-inflammation and regulation of intestinal flora disorders. RRER in the prescription plays a key role in reducing the abundance of harmful bacteria and promoting the proliferation of probiotics,which is the key of Shaoyaotang in promoting the re-balance of intestinal flora. It also confirms the scientificity of treating dampness-heat diarrhea with RRER following the therapeutic principle "treating incontinent syndrome with dredging method".
作者
凌霄
李伟霞
李春晓
王晓艳
赵娅
唐进法
李学林
LING Xiao;LI Wei-xia;LI Chun-xiao;WANG Xiao-yan;ZHAO Ya;TANG Jin-fa;LI Xue-lin(Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine/Pharmaceutical Department,The First Affiliated Hospital of Henan University of Chinese Medicine,Collaborative Innovation Center for Prevention and Treatment of Respiratory Diseases,Zhengzhou 450001,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2022年第5期16-24,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省自然科学基金项目(202300410250)
国家自然科学基金项目(82104726)
中国博士后65批面上项目(2019M652549)。
关键词
芍药汤
湿热泄泻
肠道菌群
16sDNA
大黄
Shaoyaotang
dampness-heat diarrhea
intestinal flora
16sDNA
Rhei Radix et Rhizoma
