摘要
介绍了基因编辑技术的发展和现状,并对该技术在番茄耐贮性和成熟调控、营养成分和果色、雄性不育和单性结实、逆境响应、株形、花形及野生种重新驯化等方面的研究进展进行了概述,梳理了基于CRISPR/Cas技术的单碱基编辑、DNA-free编辑及提高同源重组效率的技术创新及在番茄遗传改良和品种培育方面的应用前景和面临的挑战。
In this review,we reviewed the recent progresses of the genome editing technology and its application in tomato. We summarized successful examples in fruit storage tolerance and ripening regulation,nutrient composition and fruit color,male sterility and parthenocarpy,stress response,plant architecture,inflorescencetype and de novo domestication of wild tomato species. We further stated the technical innovations of the CRISPR/Cas-based genome editing,such as single-base editing,DNA-free editing and the improvement of homologous recombination efficiency. We finally showed the prospects and challenges in applying the genome editing technology in tomato genetic improvement and new variety breeding.
作者
张爱萍
刘江娜
闫建俊
张西英
白云凤
ZHANG Aiping;LIU Jiangna;YAN Jianjun;ZHANG Xiying;BAI Yunfeng(Institute of Agriculture Science,Sixth Dicision,Xinjiang Ptoduction and Construction Crop,Wujiaqu,Xinjiang 831300,China;College of Agriculture,Shanxi Agricultural University,Taiyuan 030031,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第1期221-232,共12页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31760571)。
关键词
基因编辑
番茄
应用
前景
genome editing
tomato
utilization
prospect
