摘要
为了研究新疆部分地区临床型乳房炎源大肠杆菌的感染率、耐药情况及致病情况,采集47份临床型乳房炎乳样进行大肠杆菌分离鉴定,并开展了药敏试验、毒力基因检测及小鼠攻毒试验。结果分离到14株大肠杆菌,分离率为29.79%(14/47)。11种抗生素药敏试验显示,对头孢哌酮+头孢曲松+红霉素+庆大霉素+强力霉素+恩诺沙星+氯霉素+万古霉素+复方新诺明+呋喃唑酮的多重耐药最为常见,高达50.00%(7/14),对阿米卡星的敏感率最高,为35.71%(5/14);14株大肠杆菌都具有多重耐药性,有71.43%(10/14)的菌株最少对9种药物具有耐药性。毒力基因检测出了Stx2、hlyA、K99、ETT2和eaeA5种毒力基因;具有多种毒力基因的菌株占28.57%(4/14)。动物毒力试验显示,试验组死亡小鼠表现为皮下充血、十二指肠菲薄、肝脏有出血点。本研究可为该地区的乳房炎源大肠杆菌的防控提供一定的帮助。
The purpose of this study was to investigate the infection rate,drug resistance and pathogenicity of clinical mastitis-derived E.coli in some areas of northern Xinjiang.The isolation and identification of 47 clinical mastitis milk samples were carried out,and drug sensitivity test,virulence gene test and mouse attack test were carried out.The results showed that 14 strains of E.coli were isolated,and the separation rate was 29.79%(14/47).The drug sensitivity test of 11 antibiotics showed that the drug resistance rate of CFP+CRO+ERY+GEN+DO+EN R+CHL+VAN+STX+FR exceeded 50.00%(7/14),and the highest sensitivity rate of amikacin was 35.71%(5/14).All 14 strains of E.coli had multiple drug resistance,and 71.43%(10/14)strains had at least 9 drug resistance.The genes detected Stx2、hlyA、K99、ETT2 and eaeA virulous genes with multiple strains,representing 28.57%(4/14).Animal virulence tests showed that the dead mice in the test group showed subcutaneous congestion,thin duodenum and bleeding points in the liver.This study can provide some help for the prevention and control of mastitis source E.coli in this area.
作者
常军帅
张琪
林津如
屈勇刚
梁晏
李娜
党瑞莹
王紫阳
CHANG Jun-shuai;ZHANG Qi;LIN Jin-ru;QU Yong-gang;LIANG Yan;LI Na;DANG Rui-ying;WANG Zi-yang(College of Animal Science and Technology,Shihezi University,Shihezi 832003;Key Laboratory of Control and Prevention of Animal Disease,Xinjiang Production&Construction Corps,Shihezi 832003)
出处
《中国奶牛》
2022年第1期21-26,共6页
China Dairy Cattle
基金
动物疾病防控兵团重点实验室开放课题(2020BTDJ03)
八师石河子市促进科技成果转化引导计划(2019ZH05)
石河子大学成果转化与技术推广计划(CGZH201908)。
