摘要
目的探究TRPC5基因对糖尿病心肌病发生发展中心肌炎症的调节作用。方法运用生物信息学方法,以TRPC5基因为靶点,预测其与其他基因的相关性及其参与的生物学过程和通路。选用TRPC5-/-和C57BL/6J♂小鼠各10只,随机分为正常组和高糖组,高糖组喂养8周,腹腔注射STZ 1周建立T2DM模型。通过HE和Masson染色观察小鼠心肌损伤情况,ELISA检测小鼠血清中肌酸激酶以及IL^(-1)β、IL-2、IL-6和IFN-γ炎症因子含量,并通过RT-PCR和Western blot分别检测IL^(-1)β、IL-2、IL-6等炎症因子及TRPC5的基因及蛋白表达。结果通过PPI互作网络发现,TRPC5基因与多种炎症基因具有相关性,且参与机体的免疫过程。病理切片结果显示,与C57BL/6J小鼠相比,TRPC5-/-组小鼠心肌损伤和免疫细胞浸润较少。RT-PCR以及血清检测结果提示,TRPC5-/-高糖组小鼠多种炎症因子在心肌中的表达量和在血清中的含量都低于C57BL/6J高糖组小鼠。结论TRPC5通过参与调节心肌细胞炎症进而影响糖尿病心肌病的发生发展。
Aim To investigate the effect of TRPC5 gene on the inflammation of diabetic cardiomyopathy.Methods The biological functions of TRPC5 and the correlation between TRPC5 gene and other genes were analyzed by bioinformatics.Studies were performed in TRPC5 knockout(TRPC5-/-)and C57 mice.Mice were randomly divided into blank control and T2DM model groups,and the model was established by intraperitoneal injection of STZ(n=10).The myocardial injury was detected by HE and Masson staining.The level of serum IL^(-1)β,IL-2,IL-6,IFN-γand creatine kinase was examined by ELISA.Gene and protein expressions of IL^(-1)β,IL-2,IL-6 and TRPC5 were analysed by RT-PCR and Western blot respectively.Results By constructing the PPI network and analyses,the TRPC5 gene was identified to interact with a variety inflammatory genes and involved in immunity.The result of pathological section showed less myocardial damage and infiltrated immune cells in TRPC5-/-mice than in C57BL/6J mice.RT-PCR and serum results showed a lower expression of inflammatory factors in myocardium and serum obtained from TRPC5 model mice than in those obtained from C57BL/6J model mice.Conclusions TRPC5 participates in the development of dilated cardiomyopathy by regulating cardiomyocyte inflammation.
作者
崔艺璇
周翔
刘宽
马学玉
肖焱波
祁艳艳
李干鹏
CUI Yi-xuan;ZHOU Xiang;LIU Kuan;MA Xue-yu;XIAO Yan-bo;QI Yan-yan;LI Gan-peng(School of Ethnic Medicine, Key Lab of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2022年第2期201-208,共8页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81760655,81960675)。
