摘要
目的探讨程序性死亡受体配体1(PD-L1)蛋白在非特殊型Ⅲ级浸润性乳腺癌中的表达及其相关因素,为免疫治疗提供依据。方法收集2017年2月至2021年2月于中国医学科学院肿瘤医院深圳医院病理明确诊断的非特殊型Ⅲ级浸润性乳腺癌手术切除标本63例。复阅HE染色切片,计算肿瘤切片内免疫细胞(IC)占所有浸润性活肿瘤细胞的比例。采用免疫组织化学方法检测所有病例标本中PD-L1(SP142)蛋白的表达情况。分析PD-L1(SP142)蛋白阳性表达与各临床病理参数间的关系,采用Pearson相关性检验分析PD-L1(SP142)表达与IC浸润程度的关系。结果63例患者中,19例(30.2%)为三阴性型,34例(53.9%)为管腔型,10例(15.9%)为人表皮生长因子受体2(HER2)过表达型。PD-L1(SP142)在非特殊型Ⅲ级浸润性乳腺癌中的阳性率为77.8%(49/63)。三阴性型中PD-L1(SP142)阳性率为94.7%(18/19),非三阴性型中为70.5%(31/44),两组间差异有统计学意义(P=0.047);雌激素受体(ER)阴性者或孕激素受体(PR)阴性者PD-L1(SP142)阳性率均为90.3%(28/31),高于ER阳性者或PR阳性者(65.6%,21/32),差异有统计学意义(P=0.018);PD-L1(SP142)阳性率与患者年龄、肿瘤发生部位、肿瘤长径及数量、脉管瘤栓、神经侵犯、淋巴结转移、HER2状态及Ki-67阳性指数均无关(均P>0.05)。PD-L1表达与IC浸润程度呈正相关(r=0.716,P<0.001)。结论非特殊型Ⅲ级浸润性乳腺癌中,三阴性型患者PD-L1阳性率较高,ER或PR阴性者PD-L1阳性率较高;肿瘤IC浸润程度与PD-L1表达正相关。
Objective To explore the expression of programmed death ligand-1(PD-L1)protein in gradeⅢnon-special type invasive breast cancer and its related factors,so as to provide a basis for immunotherapy.Methods A total of 63 surgically resected specimens of gradeⅢnon-special type invasive breast cancer diagnosed by pathology in Cancer Hospital&Shenzhen Hospital of Chinese Academy of Medical Sciences from February 2017 to February 2021 were collected.The HE-stained sections were reviewed,and the proportion of immune cells(IC)in all invasive active tumor cells in the tumor sections was calculated.The expression of PD-L1(SP142)protein in all specimens was detected by immunohistochemistry.The relationship between the positive expression of PD-L1 protein and the clinicopathological parameters was analyzed,and the Pearson correlation test was used to analyze the relationship between the expression of PD-L1(SP142)and the degree of IC infiltration.Results Among 63 patients,19 patients(30.2%)were triple-negative type,34 patients(53.9%)were luminal type,and 10 patients(15.9%)were human epidermal growth factor receptor 2(HER2)overexpression type.The positive rate of PD-L1(SP142)in gradeⅢnon-special type invasive breast cancer was 77.8%(49/63).The positive rate of PD-L1(SP142)in triple-negative type was 94.7%(18/19),the positive rate in non-triple-negative type was 70.5%(31/44),and the difference between the two groups was statistically significant(P=0.047).The positive rate of PD-L1(SP142)in estrogen receptor(ER)-negative patients or progesterone receptor(PR)-negative patients was both 90.3%(28/31),which was higher than that of ER-positive patients or PR-positive patients(65.6%,21/32),and the difference was statistically significant(P=0.018);the positive rate of PD-L1(SP142)was not related to the patient's age,tumor site,tumor maximum diameter and number,vascular tumor thrombus,nerve invasion,lymph node metastasis,HER2 status and Ki-67 positive index(all P>0.05).The expression of PD-L1 was positively correlated with the degree of IC infiltration(r=0.716,P<0.001).Conclusion In gradeⅢnon-special type invasive breast cancer,the patients with triple-negative type has a high positive rate of PD-L1,and the patient with negative ER or PR has a high positive rate of PD-L1;the tumor IC infiltration is positively correlated with the expression of PD-L1.
作者
冯莎
雷园园
沈贵华
袁丽娟
黄文亭
Feng Sha;Lei Yuanyuan;Shen Guihua;Yuan Lijuan;Huang Wenting(Department of Pathology,National Cancer Center,National Clinical Research Center for Cancer,Cancer Hospital&Shenzhen Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Shenzhen 518116,China;Department of Pathology,National Cancer Center,National Clinical Research Center for Cancer,Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China)
出处
《肿瘤研究与临床》
CAS
2021年第12期891-895,共5页
Cancer Research and Clinic
基金
国家癌症中心攀登基金(NCCN201819B70)
深圳市医疗卫生"三名工程"项目-四川大学华西医院步宏教授肿瘤病理学团队(SZSM201812076)。
