摘要
为了探讨SMC1A基因特点及其蛋白的结构和功能,通过PCR扩增SMC1A基因,将其构建至pET-28a(+)原核表达载体上,经PCR鉴定及测序鉴定后,对SMC1A基因的相关生物信息学特性进行初步探讨。结果显示:克隆获得的小鼠SMC1A基因可编码1233个氨基酸,同源性分析发现,其与大鼠源、恒河猴源和人源的SMC1A基因具有高同源性;运用软件进行SMC1A蛋白的3D建模和蛋白功能分析发现,SMC1A蛋白不存在N-糖基化位点、跨膜螺旋区域和信号肽,具有35条抗原肽和82个磷酸化位点;进行重组蛋白的Western-blot鉴定发现,pET-28a-SMC1A重组质粒所表达的蛋白会在IPTG诱导后增强。通过初步探究SMC1A基因的生物学特性、蛋白结构和功能,能够为进一步研究SMC1A基因在相关疾病中的作用机理奠定基础。
To investigate the characteristics of mouse SCM1 A gene and the structure and function of its protein,The SMC1 A gene was amplified by PCR,and cloned into pET-28 a(+)prokaryotic expression vector,the cloned coding gene was identified by PCR and sequencing,and then analyzed bioinformatics characteristics.The results indicated the cloned mouse SMC1 A gene can encode 1233 amino acids,homology analysis showed that it had high homology with SMC1 A gene from rat,rhesus monkey and human;The software was used for 3 D modeling and protein function analysis of SMC1 A protein,and it was found that there was no N-glycosylation site,transmembrane helical region and signal peptide in SMC1 A protein,but it has 35 antigenic peptides and 82 phosphorylation sites;The recombinant protein was identified by Western-blot analysis,it showed there was an increase of expression after IPTG inducement.The above results were based on the biological characteristics,protein structure and function of SMC1 A gene,the research can help to shape the foundation for study of SMC1 A-related diseases.
作者
胡丁旺
邱荣晖
傅梅萍
Hu Dingwang;Qiu Ronghui;Fu Meiping(School of Basic Medical Sciences,Fujian Medical University,Fuzhou 350122)
出处
《福建畜牧兽医》
2022年第1期30-33,38,共5页
Fujian Journal of Animal Husbandry and Veterinary medicine
基金
福建省中青年教师教育科研项目(JT180164)资助。
