摘要
目的探讨橄榄苦苷对重症急性胰腺炎大鼠心肌损伤的影响。方法25只大鼠按体重随机分成假手术组、模型组、OLE低剂量组(30 mg/kg)、OLE中剂量组(60 mg/kg)和OLE高剂量组(90 mg/kg),每组各5只。各组大鼠分别于手术造模前1 d按组别灌胃给予相应药物。造模后24 h,各组选1只大鼠进行心脏超声检查,监测大鼠造模后24 h的心脏血流动力学变化,包括左室舒张末容积(LVEDV)、左室射血分数(LVEF)和左室短轴收缩率(LVFS),每组各选3只大鼠监测心率(P),心脏血流动力学指标检测完毕后,各组大鼠分别经腹主动脉采血,分离血清,全自动生化分析仪检测血清淀粉酶、钙离子和CK-MB。结果在实验过程中,模型组、OLE低剂量组、OLE中剂量组和OLE高剂量组分别有2只大鼠于造模后24 h内死亡,假手术组大鼠未发生明显异常。与假手术组相比,左室舒张末容积(LVEDV)、左室射血分数(LVEF)和左室短轴收缩率(LVFS)三项数据模型组和OLE低剂量组变化较大;心率各组变化均不大。与假手术组血清淀粉酶浓度相比,模型组(P<0.001)、OLE低剂量组(P<0.001)、OLE中剂量组(P<0.001)和OLE高剂量组(P<0.001)均有显著性差异;与假手术组血清CK-MB浓度相比,模型组(P<0.001)、OLE低剂量组和OLE中剂量组比较,差异有统计学意义(P<0.01),而OLE高剂量组无明显差异;与模型组血清CK-MB浓度相比,OLE低剂量组(P<0.05)、OLE中剂量组(P<0.01)和OLE高剂量组(P<0.001)均有显著性差异。与假手术组血清钙离子浓度相比,模型组(P<0.001)和OLE低剂量组(P<0.01)有显著性差异,而OLE中剂量组和OLE高剂量组无差异;与模型组血清钙离子浓度相比,OLE低剂量组无显著性差异,OLE中剂量组(P<0.01)和OLE高剂量组(P<0.001)均有显著性差异。结论橄榄苦苷可改善重症急性胰腺炎大鼠心肌损伤。
Objective To investigate the effect of oleuropein on myocardial injury in rats with severe acute pancreatitis.Methods Twenty-five rats were randomly divided into the sham operation group,the model group,the OLE low-dose group(30 mg/kg),the OLE medium-dose group(60 mg/kg)and the OLE high-dose group(90 mg/kg)according to the body weight,with 5 rats in each group.The rats in each group were given corresponding drugs by intragastric administration on the day before operation.During the 24 hours after modeling,one rat in each group was selected for echocardiography examination to monitor the changes of cardiac hemodynamics in 24 hours after modeling,including the left ventricular end-diastolic volume(LVEDV),left ventricular ejection fraction(LVEF)and left ventricular fraction shortening(LVFS).Three rats in each group were selected to monitor the heart rate(P).After the cardiac hemodynamic indexes were detected,blood of the rats in each group were collected through the abdominal aorta,and their serum was separated.The serum amylase,calcium ion and CK-MB were detected by the automatic biochemical analyzer.Results During the experiment,respective two rats in the model group,the OLE low-dose group,the OLE medium-dose group and the OLE high-dose group died within 24 h after modeling,and no obvious abnormalities occurred in the sham operation group.Compared with the sham operation group,the left ventricular end-diastolic volume(LVEDV),left ventricular ejection fraction(LVEF)and left ventricular fraction shortening(LVFS)were significantly changed in the model group and the OLE low-dose group.No significant changes were observed in heart rate in each group.The serum amylase concentrations of the model group(P<0.001),the OLE low-dose group(P<0.001),the OLE medium-dose group(P<0.001)and the OLE high-dose group(P<0.001)were significantly different from that of the sham operation group.Compared with the sham operation group,statistically significant differences were observed in?the serum CK-MB concentrations of the model group(P<0.001),the OLE low-dose group(P<0.001)and the OLE medium-dose group(P<0.01),but no statistically significant difference was observed in the OLE high-dose group.Compared with the model group,statistically significant differences were observed in the serum CK-MB concentrations in the OLE low-dose group(P<0.05),OLE medium-dose group(P<0.01)and OLE high-dose group(P<0.001).Compared with the sham operation group,statistically significant differences were observed in the serum calcium concentration in the model group(P<0.001)and the OLE low-dose group(P<0.01),while no differences were observed in the OLE medium-dose group and the OLE high-dose group.Compared with the model group,no statistically significant difference was observed in the serum calcium ion concentration in the OLE low-dose group,but statistically significant differences were observed in the OLE middle-dose group(P<0.01)and the OLE high-dose group(P<0.001).Conclusion Oleuropein can relieve myocardial injury in rats with severe acute pancreatitis.
作者
杨政
章佳颖
马纪林
王洁
YANG Zheng;ZHANG Jiaying;MA Jilin;WANG Jie(Intensive Care Unit,Affiliated Hangzhou Chest Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China;Department of Nephrology,Affiliated Hangzhou Chest Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China)
出处
《中国现代医生》
2021年第33期45-49,F0003,共6页
China Modern Doctor
基金
浙江省基础公益研究计划项目(LGF19H150003)。
