摘要
目的探讨慢性乙型肝炎病毒(HBV)感染患者血清miR-122、miR-155和miR-375的表达水平及意义。方法选取2017年8月-2020年8月萍乡矿业集团有限责任公司总医院收治的60例慢性HBV感染患者为研究组,研究组患者依照病情程度分为慢性乙肝组23例、肝硬化组21例、肝癌组16例;另选取60名同期体检的健康人员为对照组。检测两组谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)指标和血清miR-122、miR-155及miR-375表达水平。采用Spearman相关性分析血清miR-122、miR-155和miR-375与病情严重程度之间的相关性。结果研究组患者ALT、AST、TBIL各肝功能指标水平和血清miR-122、miR-155和miR-375表达水平均高于对照组,差异有统计学意义(P<0.05)。肝癌组患者血清miR-122、miR-155和miR-375表达水平高于慢性乙肝组、肝硬化组,且肝硬化组患者血清miR-122、miR-155和miR-375表达水平高于慢性乙肝组,差异均有统计学意义(P<0.05)。Spearman相关性分析结果显示,CHB患者血清miR-122、miR-155和miR-375表达水平与患者病情严重程度呈正相关关系(P<0.05)。结论慢性HBV感染患者血清miR-122、miR-155及miR-375表达水平较健康人员明显上升,且与病情严重程度均呈正相关关系,参与了慢性HBV感染患者的病情进展及肝功能损伤过程。
OBJECTIVE To explore the expression levels of serum miR-122, miR-155 and miR-375 in patients with chronic hepatitis B virus(HBV) infection and analyze the significance. METHODS A total of 60 patients with chronic HBV infection who were treated in General Hospital of Pingxiang Mining Group Co., Ltd from Aug 2017 to Aug 2020 were assigned as the study group, the patients of the study group were divided into the chronic hepatitis B group with 23 cases, the liver cirrhosis group with 21 cases and the liver cancer group according to the illness condition. Meanwhile, 60 healthy people who received physical examination were chosen as the control group. The expression levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bilirubin(TBIL), serum miR-122, miR-155 and miR-375 of the two groups were detected. The association between the serum miR-122, miR-155 and miR-375 and the severity of disease was observed by means of Spearman correlation analysis. RESULTS The levels of liver function indexes ALT, AST, TBIL and expression levels of serum miR-122, miR-155 and miR-375 of the study group were significantly higher than those of the control group(P<0.05). The expression levels of serum miR-122, miR-155 and miR-375 of the liver cancer group were significantly higher than those of the chronic hepatitis B group and the liver cirrhosis group, the expression levels of serum miR-122, miR-155 and miR-375 of the liver cirrhosis group were significantly higher than those of the chronic hepatitis B group(P<0.05). Spearman correlation analysis indicated that the expression levels of serum miR-122, miR-155 and miR-375 of the CHB patients were positively correlated with the severity of disease(P<0.05). CONCLUSION The expression levels of serum miR-122, miR-155 and miR-375 of the patients with chronic HBV infection are significantly higher than those of the healthy people and are positively correlated with the severity of disease, which are involved in the progression of disease and process of liver function damage.
作者
罗艳香
程含
吴波
阳福桂
文顺平
LUO Yan-xiang;CHENG Han;WU Bo;YANG Fu-gui;WEN Shun-ping(Pingxiang Health Vocational College,Pingxiang,Jiangxi 337000,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2021年第21期3230-3234,共5页
Chinese Journal of Nosocomiology
基金
江西省教育厅科学技术研究基金资助项目(181439)。
