摘要
利用气相色谱-质谱(GC-MS)技术检测龙脑樟精油化学成分,通过1,1-二苯基苦基苯肼(DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法测定龙脑樟精油体外抗氧化能力,采用Morris水迷宫实验和免疫组化实验判断小鼠嗅吸精油后的认知功能,检测小鼠嗅吸精油后脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物(GSH-PX)和丙二醛(MDA)体内氧化指标。结果显示,龙脑樟精油鉴定出龙脑、右旋柠檬烯、桉叶素等43种主要化学成分;龙脑樟精油对DPPH和ABTS自由基的IC_(50)分别是1.65和2.27 g/L;龙脑樟精油组小鼠在Morris水迷宫中的逃避潜伏期、穿越原平台次数等指标改善,在免疫组化切片中发现β-淀粉样蛋白减少;龙脑樟精油组小鼠脑组织的SOD和GSH-PX活力提高,MDA含量减少。综上,龙脑樟精油具有一定的体外、体内抗氧化活性,对小鼠认知功能障碍具有一定的改善作用。
Gas chromatography-mass spectrometry(GC-MS)technology was used to detect chemical compositions and their relative contents of borneol essential oil.By analyzing scavenging activity of 2,2-Diphenyl-1-picrylhydrazyl(DPPH)free radical and 2,2’-azinobis[3-ethylbenzothiazoline-6-sulfonicacid]-diammonium salt(ABTS)free radical,the antioxidant activity of borneol essential oil and trolox were determined in vitro.In order to explore the effect of borneol essential oil on behavior of mice with learning and memory impairment,Morris water maze test and immunohistochemistry experiment were used to judge the cognitive function of mice in normal group,model group,borneol essential oil group and positive group.In addition,after completing the animal behavior experiment,the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-PX)and malondialdehyde(MDA)of brain tissue were measured.The results show that there are 43 main chemical components in borneol essential oil,such as borneol,d-limonene and 1,8-cineole.Free radical scavenging experiment finds that a certain concentration of borneol essential oil can effectively clean up DPPH and ABTS free radical,but its scavenging ability is less than trolox.The half maximal inhibitory concentration(IC_(50))of borneol essential oil on DPPH and ABTS free radical are 1.65 and 2.27 g/L,respectively.In Morris water maze,compared to mice in model group,the escape latency and crossing times of mice in borneol essential oil group are improved,and the performance of mice in borneol essential oil group is close to that in positive group.In immunohistochemistry experiment,the immunohistochemical section indicates that compared to mice in model group,theβ-amyloid protein of mice in borneol essential oil group decreases,and the situation of mice in borneol essential oil group is close to that in positive group.Compared to mice in model group,the activities of SOD and GSH-PX increase,and the content of MDA decreases in brain tissue of mice in borneol essential oil group.In conclusion,borneol essential oil has certain antioxidant activity in vitro and in vivo,and can improve cognitive impairment in mice.
作者
唐寅
吕晓帆
王莹
吴亚妮
Yin Tang;Xiaofan Lv;Ying Wang;Yani Wu(School of Design,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《日用化学工业》
CAS
北大核心
2021年第11期1095-1101,共7页
China Surfactant Detergent & Cosmetics
关键词
龙脑樟精油
精油成分
抗氧化
认知功能
borneol essential oil
essential oil components
antioxidation
cognitive function
