摘要
目的探讨mircoRNA基因多态性与云南汉族人群非小细胞肺癌发生发展的相关性。方法选取云南地区汉族人群非小细胞肺癌患者415例,健康对照460例,采用Taqman探针基因分型法对3个SNP位点rs2292832 T>C(pre-miR-149)、rs107822 C>T(pre-miR-219a-1),rs629367 C>A(pri-let-7a-1)进行基因分型并分析其等位基因、基因型在非小细胞肺癌患者(按病理分层为鳞癌、腺癌及其他类型肺癌;按临床分期分层为Ⅰ+Ⅱ期、Ⅲ+Ⅳ期)及健康对照中的频率差异。结果rs2292832、rs107822、rs629367位点的等位基因、基因型频率在非小细胞肺癌组和对照组间的差异没有统计学意义,在分层分析及遗传模式分析中也无显著性差异。结论rs2292832、rs107822、rs629367可能与云南汉族人群非小细胞肺癌无相关性。
Objectives To investigate the relationship between mircoRNA gene polymorphisms and the occurrence and development of non-small cell lung cancer in Yunnan Han population.Methods A total of 415 lung cancer patients of non-small cell lung cancer and 460 healthy individuals were included from Yunnan Han population.The genotypes of 3 polymorphism loci,rs2292832 T>C(pre-miR-149),rs107822 C>T(pre-miR-219 a-1),rs629367 C>A(pri-let-7 a-1)were detected by TaqmanSNP genotyping assay.The distribution of allele and genotype frequency in lung cancer patients and healthy controls were analyzed.Results There was no significant difference of the allele and genotype frequency of 3 SNPs.And in stratification analysis,there was also no significant difference.Conclusions rs2292832,rs107822,rs629367 may not be significantly associated with non-small cell lung cancer in Yunnan Han population.
作者
杨佳
李娅娴
王莹莹
肖琳
李传印
谭芳
马千里
刘舒媛
YANG Jia;LI Ya-xian;WANG Ying-ying;XIAO Lin;LI Chuan-yin;TAN Fang;MA Qian-li;LIU Shu-yuan(Dept,of Immunogenetics,Institute of Medical Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Kunming Yunnan 650118;The First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;The Third Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650118,China)
出处
《昆明医科大学学报》
CAS
2021年第10期22-28,共7页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81573206)
云南省应用基础研究计划-昆明医科大学联合专项基金资助项目(2018FE001-219,202001AY070001081)
北京协和医学院中央高校基本科研业务费资助项目(3332019111)
云南省医学后备人才项目(H-2018014)。
