摘要
目的分析寻常型银屑病皮损组织中chemerin和Sprouty1蛋白表达与病情严重程度的相关性。方法选取2018年2月-2020年11月在无锡市第五人民医院和江南大学附属医院皮肤性病科门诊接受治疗的68例寻常型银屑病患者作为实验组,以同期在两家医院皮肤性病科门诊体检的68名健康者作为对照组。采集寻常型银屑病患者躯干或四肢的皮损组织及健康者的正常皮肤组织,采用免疫组化及Western blotting检测皮肤组织中chemerin、Sprouty1蛋白相对表达量,RT-PCR检测皮肤组织中chemerin、Sprouty1 mRNA相对表达量,采用Pearson相关分析法分析chemerin、Sprouty1蛋白相对表达量与银屑病皮损面积和严重程度指数(PASI)的相关性。结果免疫组化检测结果显示,两组皮肤组织中均有chemerin、Sprouty1蛋白的表达。Chemerin蛋白在实验组皮损脂肪组织中呈高表达,在对照组皮下脂肪组织中少量表达;Sprouty1蛋白在实验组皮损颗粒层细胞膜中少量表达,在对照组表皮全层细胞的细胞膜和细胞质中呈高表达。实验组chemerin mRNA(1.478±0.14 vs. 0.858±0.08,P<0.001)和蛋白(1.138±0.12 vs. 0.789±0.06,P<0.001)相对表达量明显高于对照组,Sprouty1 mRNA(0.837±0.04 vs. 1.346±0.09,P<0.001)和蛋白(0.646±0.05 vs. 1.212±0.12,P<0.001)相对表达量明显低于对照组。Pearson相关分析显示,chemerin蛋白相对表达量与PASI评分呈正相关(r=0.536,P<0.05),Sprouty1蛋白相对表达量与PASI评分呈负相关(r=-0.619,P<0.05)。结论寻常型银屑病皮损组织中chemerin及Sprouty1蛋白相对表达量与PASI评分存在相关性。
Objective To analyze the expression of chemerin and Sprouty1 proteins in common psoriasis lesion tissues and their correlation with the severity of the disease. Methods Sixty-eight patients with common psoriasis who received treatment at the dermatology outpatient clinic of the Wuxi Fifth People’s Hospital and the Affiliated Hospital of Jiangnan University from February 2018 to November 2020 were selected as the experimental group, and another 68 healthy individuals who underwent physical examination at the dermatology outpatient clinics of the two hospitals during the same period were selected as the control group. The skin lesions of the trunk or limbs of patients with psoriasis vulgaris and normal skin tissues of healthy subjects were collected. Immunohistochemistry and Western blotting were used to detect the expression of chemerin and Sprouty1 protein in skin tissues, and RT-PCR was used to detect the relative expression of chemerin and Sprouty1 mRNA in skin tissues, and Pearsoncorrelation was used to analyze the correlation between the expression of chemerin and Sprouty1 protein with the psoriasis area and severity index(PASI). Results The results of immunohistochemical assay showed that chemerin and Sprouty1 proteins were expressed in the skin tissues of both groups. Chemerin protein was strongly expressed in the adipose tissue of the skin lesions in experimental group, and in the subcutaneous adipose tissue of the control group with a small amount of expression;Sprouty1 protein was expressed in the cell membrane of the granular layer cells of the skin lesions in experimental group with a small amount of expression, and in control group, Sprouty1 protein was strongly expressed in the cell membrane and cytoplasm of the whole epidermal layer cells. The relative expressions of chemerin mRNA(1.478±0.14 vs. 0.858±0.08, P<0.001) and protein(1.138±0.12 vs. 0.789±0.06, P<0.001) were significantly higher in experimental group than in control group, and the relative expressions of Sprouty1 mRNA(0.837±0.04 vs. 1.346±0.09, P<0.001) and protein(0.646±0.05 vs. 1.212±0.12, P<0.001) were significantly lower than that of control group. Pearson correlation analysis showed that the relative expression of chemerin protein was positively correlated with PASI score(r=0.536, P<0.05), and the relative expression of Sprouty1 protein was negatively correlated with PASI score(r=–0.619, P<0.05). Conclusion The relative expression of chemerin and Sprouty1 protein in the lesion tissue of common psoriasis correlated with PASI score.
作者
袁波
宋丹丹
吴燕
俞莺
谭城
Yuan Bo;Song Dan-Dan;Wu Yan;Yu Ying;Tan Cheng(Department of Dermatology and Venereology,Wuxi Fifth People's Hospital,Wuxi,Jiangsu 214011,China;Department of Dermatology and Venereology,Affiliated Hospital of Jiangnan University,Wuxi,Jiangsu 214000,China;Department of Physiology,School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212013,China;Department of Dermatology,Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing 210029,China)
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2021年第10期1024-1028,共5页
Medical Journal of Chinese People's Liberation Army
基金
江苏省卫计委2017年高层次卫生人才“六个一工程”拔尖人才项目(LGY2017074)。
