摘要
目的应用磁共振T2加权成像(T2-weighted imaging,T2WI)和扩散张量成像(diffusion tensor imaging,DTI)观察大脑中动脉栓塞大鼠脑组织梗死灶、水肿的形成和白质纤维的损伤,评价补阳还五汤抗脑缺血损伤的作用。方法采用线栓法制备大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)致局灶性脑缺血模型。将造模成功的大鼠按随机数字表法分为模型组、补阳还五汤组,每组5只,另取5只大鼠为假手术组。造模后24 h,补阳还五汤组大鼠灌胃补阳还五汤水煎液16.1 g/kg,连续灌胃30 d,1次/d。分别在术后3、7、14、30 d进行磁共振T2WI和DTI扫描。结果T2WI扫描结果显示,术后第3、7、14、30天,模型组大鼠大脑右侧缺血区呈异常高信号,梗死体积率随缺血时间延长逐渐降低(P<0.05);与模型组比较,术后第3、7、14、30天,补阳还五汤组大鼠梗死体积率[3 d:(13.9±13.7)%比(40.1±10.7)%;7 d:(13.9±11.9)%比(28.2±7.7)%;14 d:(10.2±7.9)%比(24.5±3.5)%;30 d:(6.8±6.5)%比(24.7±8.7)%]均明显降低(P<0.05或P<0.01);术后第3天,模型组大鼠水肿百分率达到峰值,第30天大鼠脑组织出现萎缩,水肿百分率呈负增长趋势;与模型组比较,术后第3、7天补阳还五汤组大鼠水肿百分率[3 d:(11.4%±6.9%)比(21.5%±3.1%);7 d:(5.5%±3.1%)比(8.7%±1.2%)]均明显降低(P<0.05或P<0.01)。DTI扫描结果显示,术后第3、7、14、30天,模型组梗死灶区均呈异常低信号,缺血侧皮层、纹状体rFA值均较假手术组降低(P<0.05或P<0.01)。与模型组比较,术后第14天,补阳还五汤组大鼠皮层、纹状体rFA值增加(P<0.05或P<0.01)。结论T2WI结合DTI成像可清楚提供显影病灶位置及形态,MCAO大鼠缺血区脑组织出现缺血梗死灶、水肿及白质纤维损伤,随着缺血时间的延长其病理损伤程度呈降低趋势。补阳还五汤可降低MCAO大鼠梗死体积率,减轻水肿,促进神经纤维损伤后的修复,具有一定的抗脑缺血损伤作用。
Objective This study aimed to evaluate the neuroprotective effect of Buyang-Huanwu Decoction(BYHWD)against cerebral ischemia by using magnetic T2WI and DTI to observe the infarct volume,formation of cerebral edema and injury of white matter fibers on cerebral ischemic rats.Methods Rat model of cerebral ischemia was established by middle cerebral artery occlusion(MCAO).The successfullly modeled rats were randomly divided into model group and BYHWD group,with 5 rats in each group,and the other 5 rats were taken as sham operation group.The Rats were intragastrically administrated with BYHWD(16.1 g/kg)once daily for 30 d after MCAO.T2WI and DTI examinations were performed on the 3rd,7th,14th,30th day after the surgery.Results T2WI showed abnormal hyperintensities in the right hemisphere of the MCAO rats on the 3rd,7th,14th,30th day after the surgery.The infarction percentage reduced with time coursing(P<0.05).Compared with the model group,the rats treated with BYHWD showed reduced infarction percentage[3 d:(13.9%±13.7%vs.40.1%±10.7%);7 d:(13.9%±11.9%vs.28.2%±7.7%);14 d:(10.2%±7.9%vs.24.5%±3.5%);30d:(6.8%±6.5%vs.24.7%±8.7%)](P<0.05 or P<0.01).The percentage of edema reached the peak on the 3rd day after MCAO.Due to the cerebral atrophy on 30th day,the percentage of edema exhibited negative growth.The edema percentage of BYHWD group reduced significantly on the 3rd and 7th day compared with model group[3 d:(11.4%±6.9%vs.21.5%±3.1%);7 d:(5.5%±3.1%vs.8.7%±1.2%)](P<0.05 or P<0.01).DTI showed that the low signals indicating fiber injuries were observed in the infarct areas of the model group rats on the 3rd,7th,14th,30th day after MCAO.The rFA values in the ipsilateral cortex and striatum of MCAO rats were significantly decreased compared with the sham group(P<0.05 or P<0.01).The rFA values in cortex and striatum in focal cerebral ischemia rats of BYHWD group were higher than those in the model group,while only 14 d showed significance(P<0.05 or P<0.01).Conclusions T2WI and DTI clearly provided the informations of the location and morphology of the infarct areas.The ischemic brain showed significant infarction,edema,and white matter injury,which were ameliorated with time going on.BYHWD reduced the infarction percentage,inhibited cerebral edema and stimulated the recovery of neurofibra,suggesting that BYHWD could protect against cerebral ischemia.
作者
王晶晶
吴娜
李慢中
卢静
Wang Jingjing;Wu Nang;Li Manzhong;Lu Jing(Laboratory Animal Department,Capital Medical University,Beijing 100069,China;Department of Pharmacy,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)
出处
《国际中医中药杂志》
2021年第9期873-879,共7页
International Journal of Traditional Chinese Medicine
基金
北京市自然科学基金(7212161、7172034)。
关键词
补阳还五汤
脑缺血
磁共振成像
大鼠
Bu Yang Huan Wu Tang
Brain ischemia
Magnetic resonance imaging
Rats
