摘要
目的研究体感诱发电位(SEP)的时频特征及其对山羊颈脊髓损伤模型的诊断价值。方法选择12只3~6个月龄山羊,体质量15~20 kg。采用球囊注射压迫法制备慢性颈脊髓压迫损伤模型;使用诱发电位记录系统(YRKJA2004),记录颈脊髓损伤前后的SEP信号。应用匹配追踪算法提取SEP信号的时频成分,分析SEP信号时域及时频域特征参数,并比较颈脊髓损伤前后SEP时频成分特征及分布模式。结果所有动物造模后均出现功能障碍,颈脊髓受到轻度损伤,造模成功。颈脊髓损伤后,SEP信号的时域波形潜伏期延长(正常组11.39 ms±0.87 ms;损伤组13.56 ms±2.04 ms),波幅降低(正常组1.38μV±0.59μV;损伤组0.57μV±0.35μV)。同时,SEP信号的时频成分存在稳定的分布模式。颈脊髓损伤前,SEP信号在时频域存在1个稳定的主成分(16~22 ms、65~90 Hz)和2个次成分(次成分1:20~28 ms,150~210 Hz;次成分2:35~44 ms,100~210 Hz);与损伤前相比,损伤后SEP时频成分的分布区域存在显著差异(主成分:19~29 ms,60~120 Hz;次成分1:27~36 ms,160~230 Hz;次成分2:37~48 ms,40~150 Hz),并且时频成分的数量增加,出现了新的次成分,分布模式发生显著变化,且变化差异有统计学意义(P <0.05)。结论高分辨率SEP时频分析能提供反映颈脊髓损伤的相关信息,将有望为颈脊柱脊髓疾病的病理严重程度准确评估和精准的病灶定位诊断提供一种新思路。
Objective To evaluate time-frequency characteristics and value of somatosensory evoked potentials(SEP) in diagnosis of goat cervical spinal cord injury model. Methods Twelve goats, aged 3-6 months, body weight of 5-20 kg were selected as experimental animal. The chronic spinal cord injury model was established by balloon injection compression method.The evoked potential recording system(YRKJ-A2004) was used to record SEP signals before injury(normal group) and after injury(injury group). The matching pursuit algorithm was used to extract time-frequency components(TFC) of SEP signals. The characteristic parameters of SEP signal in time domain and time-frequency domain were analyzed and distribution patterns of TFC before and after injury were compared. Results All animals showed dysfunction after modeling, the cervical spinal cord was slightly injured, and the modeling was successful. After spinal cord injury, the latency of time-domain waveform of SEP signal was prolonged(normal group 11.39 ms ± 0.87 ms;injury group 13.56 ms ± 2.04 ms), amplitude was reduced(normal group 1.38 μV ± 0.59 μV;injury group 0.57 μV ± 0.35 μV). Simultaneously, the TFC of SEP signal had stable distribution pattern. Before spinal cord injury, SEP signal had one stable main TFC(16-22 ms, 65-90 Hz) and two sub-TFC in timefrequency domain(sub-TFC1: 20-28 ms, 150-210 Hz;sub-TFC2: 35-44 ms, 100-210 Hz). Compared with that before injury, the distribution area of TFC of SEP after injury was significantly different(main TFC: 19-29 ms, 60-120 Hz;sub-TFC1:27-36 ms, 160-230 Hz;sub-TFC2: 37-48 ms, 40-150 Hz), numbers of TFC were increased and novel subcomponents was observed. The distribution pattern changed significantly, which was statistically significant(P < 0.05). Conclusion It is demonstrated that high-resolution time-frequency analysis of SEP could provide relevant information reflecting spinal cord injury, which will provide novel ideas for accurate assessment of pathology stage and precise foci localization for cervical spine cord diseases.
作者
吴怡欣
谢小波
胡勇
崔红岩
WU Yi-xin;XIE Xiao-bo;HU Yong;CUI Hong-yan(Institute of Biomedical Engineering,Chinese A-cademy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China;Department of Orthopaedics and Traumatology,The University of Hong Kong,Hong Kong,China)
出处
《生物医学工程与临床》
CAS
2021年第5期527-532,共6页
Biomedical Engineering and Clinical Medicine
基金
国家自然科学基金资助项目(81871768)
天津市自然科学基金资助项目(18JCYBJC29600)
广东省高水平医院建设科研培育计划项目(HKUSZH201902011)。
关键词
脊髓型颈椎病
慢性颈脊髓压迫损伤模型
体感诱发电位
时频分析
匹配追踪
羊
cervical spondylotic myelopathy
chronic cervical spinal cord compression injury model
somatosensory evoked potentials
time-frequency analysis
matching pursuit
goat
